Multiphoton excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein
Multiphoton excitation-evoked chromophore-assisted laser inactivation using green fluorescent protein
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DOI：
10.1038/nmeth770
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发表时间：
2005-07-01
期刊：
影响因子：
48
通讯作者：
Takamatsu, T
中科院分区：
文献类型：
--
作者：
Tanabe, T;Oyamada, M;Takamatsu, T
需要非侵入性的、直接的方法来以高时空分辨率在活细胞中检测选定的蛋白质。发色团辅助激光灭活（卡利）可用于光化学灭活蛋白质，但它有一些缺点，如程序复杂和非特异性光损伤。在这里，我们表明，通过应用多光子激发卡利，增强型绿色荧光蛋白（EGFP）是一种有效的发色团的蛋白质的功能失活没有非特异性的光损伤在活的哺乳动物细胞。
Noninvasive, straightforward methods to inactivate selected proteins in living cells with high spatiotemporal resolution are needed. Chromophore-assisted laser inactivation (CALI) can be used to photochemically inactivate proteins, but it has several drawbacks, such as procedural complexity and nonspecific photodamage. Here we show that by application of multiphoton excitation to CALI, enhanced green fluorescent protein (EGFP) is an effective chromophore for inactivation of a protein's function without nonspecific photodamage in living mammalian cells.