Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously
Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously
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DOI：
10.1023/a:1008341128327
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Tjandra, N
中科院分区：
文献类型：
--
作者：
de Alba, E;Suzuki, M;Tjandra, N
为了测量剩余的偶极偶联，所研究的分子必须在磁场存在下部分定向。已经观察到一些蛋白质样品在取向介质施加的条件下是不稳定的。如果连续测量不同类型的偶极耦合，它们的值将不符合随时间缓慢变化的唯一对准张量。这可能会使结构计算产生偏差。同时获得不同类型的偶极耦合更为合适，这样所有数据对应于一个有效对准张量。我们在这里描述了一种通用的核磁共振策略，它可以适应各种现有的脉冲序列。
In order to measure residual dipolar couplings, the molecule under study has to be partially oriented in the presence of the magnetic field. It has been observed that some protein samples are not stable under the conditions imposed by the orienting media. If different types of dipolar couplings are measured sequentially, their values will not agree with a unique alignment tensor that is changing slowly over time. This could bias the structure calculation. It would be more appropriate to obtain different types of dipolar couplings simultaneously, such that all the data correspond to one effective alignment tensor. We describe here a general NMR strategy designed to do so, that can be adapted to various existing pulse sequences.