Interaction of enteroaggregative Escherichia coli with salad leaves
Interaction of enteroaggregative Escherichia coli with salad leaves
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DOI：
10.1111/j.1758-2229.2009.00037.x
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发表时间：
2009-08-01
影响因子：
3.3
通讯作者：
Frankel, Gad
中科院分区：
文献类型：
--
作者：
Berger, Cedric N.;Shaw, Robert K.;Frankel, Gad
肠聚集性大肠杆菌（EAEC）是人类重要的病原体。然而，其环境水库是未知的。随着新鲜沙拉叶越来越被认为是人类病原体的重要环境载体，我们研究了EAEC菌株的叶附着能力。我们发现临床EAEC分离物与Eruca vesicaria（通常称为火箭或芝麻菜）叶片的结合可分为高、中和低粘附表型。使用原型EAEC菌株042调查叶片附着所涉及的划线机制，我们发现小附着细菌聚集在整个叶片表面和密集的细菌附着到气孔的保卫细胞。aaf 042突变体失去了结合表皮的能力，同时保持气孔粘附。相反，fliC 042突变体保留了结合表皮的能力，但失去了气孔向性。这些结果表明，多种粘附因子参与了EAEC与叶片的相互作用，EAEC使用类似的定殖因子来结合粘膜和叶片表面，新鲜农产品可能是EAEC菌株的重要储存库。
Enteroaggregative Escherichia coli (EAEC) are important human pathogens. However, their environmental reservoir is unknown. As fresh salad leaves are increasingly recognized as an important environmental vector for human pathogens, we investigated leaf attachment capability of EAEC strains. We found that binding of clinical EAEC isolates to leaves from Eruca vesicaria (commonly known as rocket or arugula) can be divided into high, moderate and low adherent phenotypes. Using the prototype EAEC strain 042 to investigate the underlining mechanisms involved in leaf attachment, we found small attached bacterial aggregates over the entire leaf surface and dense bacterial attachment to the guard cell of the stomata. An aaf 042 mutant lost the ability to bind the epidermis while retaining stomatal adherence. In contrast, a fliC 042 mutant retained the ability to bind the epidermis but lost stomatal tropism. These results show that multiple adherence factors are involved in the interaction of EAEC with leaves, that EAEC uses similar colonization factors to bind mucosal and leaf surfaces and that fresh produce might be an important reservoir of EAEC strains.