Influence of induced heat stress on HSP70 in buffalo lymphocytes.
Influence of induced heat stress on HSP70 in buffalo lymphocytes.
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DOI：
10.1111/j.1439-0396.2010.01082.x
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发表时间：
2011-08
影响因子：
2.7
通讯作者：
A. Mishra;O. K. Hooda;G. Singh;S. K. Meur
中科院分区：
文献类型：
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作者：
A. Mishra;O. K. Hooda;G. Singh;S. K. Meur
夏季和夏季后季节期间农场动物（例如牛和布法罗）的热应激对于畜牧生产者来说是一个问题。当热应激伴随环境湿度损害动物的免疫状态、生长、生产和繁殖性能时，热应激的影响变得明显。在不同应激源存在下，细胞培养物中HSP70水平的增加通常并不反映动物控制热调节的生理适应性。本研究直接比较了不同热应激条件对布法罗体内、外淋巴细胞免疫状态和HSP 70表达的影响。将Murrah布法罗犊牛置于心理测试箱中，每天4 h，连续12 d，并与自然条件下饲养在实验棚中的对照组动物进行比较。与对照组相比，在两种热应激条件下血清HSP 70水平均增加>200倍。此外，犊牛的免疫状态不能激活血清淋巴细胞中HSP 70的表达水平。体外培养的淋巴细胞在较高温度下产生2.5倍的HSP70浓度增加。本研究首次证明了与体外处理的细胞相比，布法罗血清HSP 70水平作为热适应性反应的更复杂的表达模式。本研究的结果表明，血清HSP 70水平可以作为一个敏感的生物标志物热应激管理在大型农场动物。
Heat stress in farm animals, such as cattle and buffalo during summer and post-summer seasons is a problem for livestock producers. The effect of heat stress becomes pronounced when heat stress is accompanied with ambient humidity impairing the immune status, growth, production and reproductive performance of animals. Increase in HSP70 levels from cell cultures in presence of different stressors often does not reflect the physiological adaptability of animals governing thermal regulation. In this study we directly compared the effect of different heat stress conditions with the immune status and HSP70 expression patterns from buffalo lymphocytes both in vivo and in vitro. Murrah buffalo calves were exposed to induced heat stress with two experimental treatments: hot-dry (42 °C with existing relative humidity) or hot humid (35 °C with 70% relative humidity) condition in psychometric chamber, 4 h daily for 12 days and compared with control animals maintained in an experimental shed under natural conditions. There was >200-fold increase in serum-HSP70 levels in both heat stress conditions compared with control. Furthermore, the immune status of the calves failed to activate the level of HSP70 expression in serum lymphocytes. Lymphocytes cultured in vitro at higher temperature exert 2.5-fold increase in HSP70 concentration. This study is the first of its kind to demonstrate more complex expression pattern of buffalo serum-HSP70 level as a thermo adaptive response compared with in vitro treated cells. Results from this study indicate that serum-HSP70 levels could be used as a sensitive biomarker for heat stress management in large farm animals.