Effect of the glycocalyx layer on transmission of interstitial flow shear stress to embedded cells.
Effect of the glycocalyx layer on transmission of interstitial flow shear stress to embedded cells.
复制标题
糖萼层对间质流动剪切应力向嵌入细胞传递的影响。
DOI：
10.1007/s10237-012-0385-8
复制
发表时间：
2013-01
影响因子：
3.5
通讯作者：
Shi, Zhong-Dong
中科院分区：
文献类型：
--
作者：
Tarbell, John M.;Shi, Zhong-Dong
在本文中，开发了一个简单的理论模型来描述从间质液流到被蛋白聚糖/糖蛋白层（糖萼）覆盖并嵌入细胞外基质中的细胞表面的力传递。 Brinkman 方程用于描述通过细胞外基质和糖萼层的流动，并确定由流体流动载荷在糖萼中产生的固体机械应力。使用细胞外基质和糖萼层的达西渗透率以及间质流速的合理值，我们能够估计体内和体外对嵌入的血管、软骨和肿瘤细胞表面施加的流体和固体剪切应力。主要发现是表面固体应力通常比表面流体应力大一到两个数量级。这表明细胞表面糖萼可以感知间质流剪切应力，支持了最近的许多观察结果，即间质流可以诱导嵌入细胞中的机械转导。这项研究可能有助于理解胚胎发生、肿瘤发生、组织生理学和疾病中与间质流相关的力学生物学，并对组织工程具有重要意义。
In this paper, a simple theoretical model is developed to describe the transmission of force from interstitial fluid flow to the surface of a cell covered by a proteoglycan / glycoprotein layer (glycocalyx) and embedded in an extracellular matrix. Brinkman equations are used to describe flow through the extracellular matrix and glycocalyx layers and the solid mechanical stress developed in the glycocalyx by the fluid flow loading is determined. Using reasonable values for the Darcy permeability of extracellular matrix and glycocalyx layers and interstitial flow velocity, we are able to estimate the fluid and solid shear stresses imposed on the surface of embedded vascular, cartilage and tumor cells in vivo and in vitro. The principal finding is that the surface solid stress is typically one to two orders of magnitude larger than the surface fluid stress. This indicates that interstitial flow shear stress can be sensed by the cell surface glycocalyx, supporting numerous recent observations that interstitial flow can induce mechanotransduction in embedded cells. This study may contribute to understanding of interstitial flow-related mechanobiology in embryogenesis, tumorigenesis, tissue physiology and diseases and has implications in tissue engineering.
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DOI：
10.1016/j.bbrc.2007.01.137
发表时间：
2007-03-30
影响因子：
3.1
作者：
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