Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions
Varietal differences in flavonoid and antioxidant activity in Japanese soybean accessions
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DOI：
10.1093/bbb/zbaa104
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发表时间：
2021-03-24
影响因子：
1.6
通讯作者：
Akashi, Ryo
中科院分区：
文献类型：
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作者：
Arifin, Hana Akari;Hashiguchi, Takuyu;Akashi, Ryo
大豆种子含有许多抗氧化剂，包括类黄酮和其他植物化学物质。异黄酮是一种植物雌激素，可模仿雌激素对靶组织的作用，并通过隔离自由基发挥抗氧化活性。尽管迄今为止已开发出许多品种，但日本大豆品种中类黄酮含量和抗氧化活性的品种差异仍不清楚。在这里，我们评估了 26 个大豆品种中异黄酮、总黄酮和总酚的种子含量。接下来，使用与人 HepG2 稳定细胞中的荧光素酶报告基因连接的抗氧化反应元件 (ARE) 检查每个品种的抗氧化活性。所有大豆种质的相对ARE荧光素酶活性率变化达4倍，范围为1.00至4.02； 22 个品种表现出显着的抗氧化活性。相关分析表明总异黄酮水平与抗氧化活性呈中度相关。
Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues and also exerts antioxidant activity by sequestering free radicals. Despite many cultivars developed to date, varietal differences in flavonoid content and antioxidant activity in Japanese soybean accessions remain less well characterized. Here, we evaluated the seed content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions. Next, the antioxidant activity of each accession was examined using antioxidant responsive element (ARE) linked to a luciferase reporter in human HepG2 stable cells. The relative ARE luciferase activity rate of all soybean accessions varied up to 4-fold which ranged from 1.00 to 4.02; and 22 accessions exhibited significant antioxidant activities. Correlation analysis indicated that the level of total isoflavone moderately correlated with antioxidant activity.