The insulin signaling pathway in honey bee (Apis mellifera) caste development -: differential expression of insulin-like peptides and insulin receptors in queen and worker larvae
The insulin signaling pathway in honey bee (Apis mellifera) caste development -: differential expression of insulin-like peptides and insulin receptors in queen and worker larvae
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DOI：
10.1016/j.jinsphys.2008.04.009
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Hartfelder, Klaus
中科院分区：
文献类型：
--
作者：
de Azevedo, Sergio Vicente;Hartfelder, Klaus
胰岛素/胰岛素样信号通路（IIS）是后生动物中控制身体大小和相关器官生长的进化保守模块。在高度社会化的蜜蜂中，种姓表型不仅在大小和几个结构特征上不同，而且在个体适应性和生活史上也不同。我们研究了蜜蜂基因组中预测的两种胰岛素样肽（AmILP-1和AmILP-2）和两种胰岛素受体（AmInR-1和AmInR-2）编码基因的发育表达谱。在种姓发展的关键阶段，女王和工人幼虫的定量PCR分析表明，AmILP-2是占主导地位的转录ILP在两个种姓，与更高的表达在工人比在女王。这两个InR基因的表达急剧下降，在四龄女王幼虫，但在工人几乎没有调制。乍一看，这些发现是非直观的，考虑到皇后的较高的增长率，但它们可以被解释为IIS模块和保幼激素之间可能的拮抗串扰。分析AmInR-1和AmInR-2在女王和工人的幼虫卵巢中的表达揭示了低的转录水平在女王和AmInR-2的表达在五龄工人的幼虫急剧下降，表明相对独立的组织特异性与整体IIS途径的活动。(C)2008爱思唯尔有限公司保留所有权利。
The insulin/insulin-like signaling (IIS) pathway is an evolutionarily conserved module in the control of body size and correlated organ growth in metazoans. In the highly eusocial bees, the caste phenotypes differ not only in size and several structural features but also in individual fitness and life history. We investigated the developmental expression profiles of genes encoding the two insulin-like peptides (AmILP-1 and AmILP-2) and the two insulin receptors (AmInR-1 and AmInR-2) predicted in the honey bee genome. Quantitative PCR analysis for queen and worker larvae in critical stages of caste development showed that AmILP-2 is the predominantly transcribed ILP in both castes, with higher expression in workers than in queens. Expression of both InR genes sharply declined in fourth instar queen larvae, but showed little modulation in workers. On first sight, these findings are non-intuitive, considering the higher growth rates of queens, but they can be interpreted as possibly antagonistic crosstalk between the IIS module and juvenile hormone. Analyzing AmInR-1 and AmInR-2 expression in ovaries of queen and worker larvae revealed low transcript levels in queens and a sharp drop in AmInR-2 expression in fifth instar worker larvae, indicating relative independence in tissue-specific versus overall IIS pathway activity. (C) 2008 Elsevier Ltd. All rights reserved.