Differential expression of keratins 10, 17, and 19 in normal cervical epithelium, cervical intraepithelial neoplasia, and cervical carcinoma
Differential expression of keratins 10, 17, and 19 in normal cervical epithelium, cervical intraepithelial neoplasia, and cervical carcinoma
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DOI：
10.1136/jcp.52.1.41
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发表时间：
1999-01-01
影响因子：
3.4
通讯作者：
Hanby, A
中科院分区：
文献类型：
--
作者：
Maddox, P;Sasieni, P;Hanby, A
目的-为了检查免疫组化的价值，在定义角蛋白的配置文件，以帮助宫颈组织病理学diagnosis. Methods-免疫组化定位角蛋白17，10，和19进行了研究，在268宫颈活检216名妇女，包括正常上皮细胞（有和没有人乳头状瘤病毒），低和高级别的宫颈上皮内瘤变，和浸润性癌。阳性免疫染色的百分比进行了评分，使用Kontron MOP视频计划图像analysis.Results-All主要类别的宫颈上皮细胞表达这些角蛋白在不同程度上。角蛋白10在正常组织中的免疫染色百分比中位数为40%，而在浸润性癌中仅为1%（p < 0.0001）。角蛋白17的中位数在正常组中为0%，在癌中为80%（p < 0.0001）。相比之下，角蛋白19的染色没有显著差异。使用角蛋白10和17百分比的组合，可以将癌与良性疾病分开，灵敏度为100%，特异性为93%。组内的进一步分析显示，在储备细胞增生，不成熟的鳞状上皮化生，和先天性transformation zone. Conclusions，在宫颈内的形态多样性，反映了更广泛的染色角蛋白10和19，在一定程度上，由不同的角蛋白模式。角蛋白10和17在浸润性鳞状细胞癌和正常宫颈上皮中的表达模式有显著差异。
Aim-To examine the value of immunohistochemistry in defining a keratin profile to aid cervical histopathological diagnosis.Methods-Immunohistochemical localisation of keratins 17, 10, and 19 was studied in 268 cervical biopsies from 216 women including normal epithelia (with and without human papilloma virus), low and high grade cervical intraepithelial neoplasia, and invasive carcinoma. The percentage of positive immunostaining was scored using a Kontron MOP video-plan image analyser.Results-All major categories of cervical epithelia expressed these keratins to varying degrees. The median percentage of immunostaining for keratin 10 was 40% in normal tissue compared with just 1% in invasive carcinoma (p < 0.0001). The medians for keratin 17 were 0% in the normal group and 80% in carcinomas (p < 0.0001). By contrast, there was no significant difference in staining for keratin 19. Using a combination of the keratin 10 and 17 percentages, it was possible to separate the carcinomas from the benign conditions with a sensitivity of 100% and a specificity of 93%. Further analyses within the groups revealed more extensive staining for keratins 10 and 19 in reserve cell hyperplasia, immature squamous metaplasia, and congenital transformation zone.Conclusions-The morphological variety within the cervix is reflected, in part, by distinct keratin patterns. There are striking differences in the patterns of keratins 10 and 17 between infiltrating squamous carcinoma and normal cervical epithelia.