Expression and significance of lysyl oxidase-like 1 and fibulin-5 in the cardinal ligament tissue of patients with pelvic floor dysfunction.
Expression and significance of lysyl oxidase-like 1 and fibulin-5 in the cardinal ligament tissue of patients with pelvic floor dysfunction.
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DOI：
10.7555/jbr.27.20110142
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发表时间：
2013-01
影响因子：
2.3
通讯作者：
Bo L
中科院分区：
文献类型：
--
作者：
Zhou Y;Ling O;Bo L
盆腔器官脱垂（POP）是一种女性致残性疾病，其特征是失去盆底支撑，导致子宫突出进入或通过阴道。POP是一个复杂的问题，可能涉及多种机制，可用的治疗方法有限，并且与包括弹性纤维在内的结缔组织缺陷有关。本研究旨在研究POP组与非POP组在主韧带中纤维蛋白-5和赖氨酸氧化酶样1 （LOXL1）的表达。在经腹子宫切除术中，从53名患有POP的妇女和25名年龄和胎次匹配的绝经后良性妇科病理的非POP妇女的主韧带中获得标本。免疫组织化学染色法检测蛋白表达。统计分析采用统计学软件包SPSS13.0系统进行卡方检验和Spearman相关分析。结果显示，与非POP组相比，POP组主韧带中fibulin-5和LOXL1的表达均降低（P < 0.05）。fibulin-5、LOXL1的表达与POP分期、伴应激性尿失禁、阴道分娩次数密切相关（P < 0.05），与绝经后状态无关（P < 0.05）。fibulin-5在POP中的表达与LOXL1呈正相关（P < 0.05）。我们认为纤维蛋白-5和LOXL1表达的变化可能在POP的发生中起作用。
Pelvic organ prolapse (POP) is a disabling disorder in women characterized by a loss of pelvic floor support, leading to the herniation of the uterus into or through the vagina. POP is a complex problem that likely involves multiple mechanisms with limited therapies available, and is associated with defects in connective tissue including elastic fibers. This study was designed to investigate the expression of fibulin-5 and lysyl oxidase-like 1 (LOXL1) in the cardinal ligament in samples taken from the POP group compared to the non-POP group. Specimens were obtained during abdominal hysterectomy from the cardinal ligament of 53 women with POP and 25 age- and parity- matched women with non-POP among post-menopausal women with benign gynecologic pathology. Protein expression was evaluated using the immunohistochemical staining method. For statistical analyses, chi-square test and Spearman's correlation were used with the statistical package SPSS13.0 system. Our results showed that both fibulin-5 and LOXL1 expressions were decreased in the cardinal ligament in the POP group compared to the non-POP group (P < 0.05). The expression of fibulin-5 and LOXL1 were correlated closely with the stage of POP, accompanied by stress urinary incontinence and frequency of vaginal delivery (P < 0.05), but had no relationship with post-menopausal state (P > 0.05). The expression of fibulin-5 was positively associated with LOXL1 in POP (P < 0.05). We conclude that changes in fibulin-5 and LOXL1 expression may play a role in the development of POP.