Frequency response properties of primary afferent neurons in the posterior lateral line system of larval zebrafish
Frequency response properties of primary afferent neurons in the posterior lateral line system of larval zebrafish
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DOI：
10.1152/jn.00414.2014
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发表时间：
2015-01-15
影响因子：
2.5
通讯作者：
Liao, James C.
中科院分区：
文献类型：
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作者：
Levi, Rafael;Akanyeti, Otar;Liao, James C.
鱼类用其侧线系统的神经突探测水流的能力取决于传入神经元的生理以及水动力环境。利用斑马鱼幼鱼（Danio rerio），我们测量了初级传入神经元对单个浅表神经突机械偏转的基本反应特性。我们使用了两种刺激方案。首先，我们使用正弦波刺激来表征传入神经元的响应特性。在0 ~ 100 Hz的刺激频率范围内，平均频率响应曲线是平坦的，与位移检测器的滤波特性相匹配。峰值速率随频率渐近增加，锁相在10 ~ 60 Hz之间达到最大值。其次，我们使用脉冲序列刺激来分析最大尖峰速率能力。我们发现传入神经元可以产生高达80个脉冲/s，并且可以可靠而精确地跟随高达40个脉冲/s的脉冲序列刺激速率。正弦波和脉冲刺激方案都表明，传入神经元在低频率刺激下比在高频率刺激下维持其诱发活动的持续时间更长。我们发现了一种基于自发活动模式的传入神经元，并发现了自发活动水平与诱发活动之间的相关性。总的来说，我们的研究结果建立了斑马鱼幼体侧线初级传入神经元的基线反应特性，这是理解脊椎动物机械感受系统如何感知和随后处理来自环境的信息的关键一步。
The ability of fishes to detect water flow with the neuromasts of their lateral line system depends on the physiology of afferent neurons as well as the hydrodynamic environment. Using larval zebrafish (Danio rerio), we measured the basic response properties of primary afferent neurons to mechanical deflections of individual superficial neuromasts. We used two types of stimulation protocols. First, we used sine wave stimulation to characterize the response properties of the afferent neurons. The average frequency-response curve was flat across stimulation frequencies between 0 and 100 Hz, matching the filtering properties of a displacement detector. Spike rate increased asymptotically with frequency, and phase locking was maximal between 10 and 60 Hz. Second, we used pulse train stimulation to analyze the maximum spike rate capabilities. We found that afferent neurons could generate up to 80 spikes/s and could follow a pulse train stimulation rate of up to 40 pulses/s in a reliable and precise manner. Both sine wave and pulse stimulation protocols indicate that an afferent neuron can maintain their evoked activity for longer durations at low stimulation frequencies than at high frequencies. We found one type of afferent neuron based on spontaneous activity patterns and discovered a correlation between the level of spontaneous and evoked activity. Overall, our results establish the baseline response properties of lateral line primary afferent neurons in larval zebrafish, which is a crucial step in understanding how vertebrate mechanoreceptive systems sense and subsequently process information from the environment.