Response of stem cells from human exfoliated deciduous teeth (SHED) to three bioinductive materials - An in vitro experimental study
Response of stem cells from human exfoliated deciduous teeth (SHED) to three bioinductive materials - An in vitro experimental study
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DOI：
10.1016/j.sdentj.2019.05.005
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发表时间：
2020-01-01
影响因子：
1.8
通讯作者：
Bogar, Chetana
中科院分区：
文献类型：
--
作者：
Dahake, Prasanna T.;Panpaliya, Nikita P.;Bogar, Chetana
简介：干细胞具有无与伦比的再生能力和潜能，当单独使用或与支架材料联合使用时，可分化为任何成熟细胞。目的：评价EMD的功能分化潜能（釉质基质衍生物），MTA（矿物三氧化物聚集体）和生物牙本质对人类脱落乳牙干细胞（SHED）的影响。目的：确定功能分化潜能材料和方法：将传代至第5代的SHED细胞分别用EMD、MTA和Biodentine处理，用MTT法比较和评价其对细胞活力的影响（3- 4，5-二甲基噻唑-2-基-2，5-二苯基四唑溴化物）测定7天。茜素红S染色用于通过染色钙沉积物14天来评估这些材料的矿化潜力。结果：观察到EMD在7天结束时赋予最高的细胞活力（p < 0.001），其次是Biodentine和MTA。同样，EMD在14天结束时显示出最高的矿化和牙本质涎蛋白表达的潜力（p < 0.001），其次是生物牙本质和MTA（p <0.001）。
Introduction: Stem cells have unmatched capacity and potential for regeneration and when used alone or in combination with scaffolds to replace or repair damaged cells, can differentiate into any mature cell.Aim: To evaluate the functional differentiation potential of EMD (Enamel Matrix Derivative), MTA (Mineral Trioxide Aggregate) and Biodentine on Stem Cells from Human Exfoliated Deciduous teeth (SHED).Objective: To determine functional differentiation potential (osteogenic/odontogenic) of various biomaterials on SHED.Material and method: SHED derived from 5th linear passage after sub-culturing were treated with EMD, MTA and Biodentine individually and their effect on cell viability was compared and evaluated by MTT (3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) assay for 7 days. Alizarin red S staining was used to assess mineralization potential of these materials by the staining calcium deposits for 14 days. The results were analyzed using One-way ANOVA, Post hoc Tukey's test for multiple comparisons.Results: It was observed that EMD imparted the highest cell viability at the end of 7 days (p < 0.001) followed by Biodentine and MTA. Likewise EMD showed highest potential to enhanced mineralization and expression of dentine sialoprotein (p < 0.001) followed by Biodentine and MTA at the end of 14 days (p