Centrifuge Microscopy to Analyze the Sedimentary Movements of Amyloplasts

Centrifuge Microscopy to Analyze the Sedimentary Movements of Amyloplasts
复制标题

离心显微镜分析淀粉体的沉积运动

DOI：
10.21769/bioprotoc.1229
复制
发表时间：
2014
期刊：
--
影响因子：
--
通讯作者：
M. Morita
M. Morita
中科院分区：
--
文献类型：
--
作者：
M. Toyota;Norifumi Ikeda;M. Tasaka;M. Morita

文献摘要

被引文献

相似文献

[摘要]离心显微镜（CMS）功能上由产生离心力（超重力条件）的离心机和放大物体图像的显微镜组成。这种设备组合允许在离心过程中进行活细胞成像。我们开发了一种新的CMS（NSK Ltd.）为了观察植物细胞器如造淀粉体在超重力条件下的运动（Toyota等人，2013年）。该CMS在时空分辨率、易用性和紧凑性方面不同于先前设计的CMS。在这里，我们展示了一个快速的协议，准备样本的拟南芥花序茎，使用CMS，获得成像数据，并分析它们使用一个单一的跟踪方法。
[Abstract] A centrifuge microscope (CMS) functionally consists of a centrifuge producing a centrifugal force (hypergravity condition) and a microscope making an enlarged image of an object. This combination of equipment allows live-cell imaging during centrifugation. We have developed a new CMS (NSK Ltd.) to observe movements of the plant organelles such as amyloplasts, under hypergravity conditions (Toyota et al., 2013). This CMS is distinct from previously designed CMSs in terms of spatio-temporal resolution, ease of use and compactness. Here, we show a quick protocol to prepare a specimen of Arabidopsis inflorescence stem, use the CMS, obtain imaging data and analyze them using a single tracking method.