Chriz, a chromodomain protein specific for the interbands of Drosophila melanogaster polytene chromosomes
Chriz, a chromodomain protein specific for the interbands of Drosophila melanogaster polytene chromosomes
复制标题
DOI：
10.1007/s00412-005-0339-3
复制
发表时间：
2005-05-01
期刊：
影响因子：
1.6
通讯作者：
Saumweber, H
中科院分区：
文献类型：
--
作者：
Gortchakov, AA;Eggert, H;Saumweber, H
多线间期染色体被压缩成一系列的带和带间，反映它们被组织成独立的染色体结构域。为了了解染色体组织，我们着手研究对带间有选择性的蛋白质的作用。在这里，我们描述了果蝇chromodomain蛋白Chriz是coimmunoprecipitated与锌指蛋白Z4。这两种蛋白质共定位于果蝇多线染色体上的带间区。像Z4一样，Chriz在整个发育过程中普遍表达，并与所有间期核中的染色质相关。在有丝分裂早期，Chriz与染色质分离后，与中心体和有丝分裂纺锤体结合。新诱导的无定形Chriz等位基因是早期致死的，并且Chriz的普遍过表达也是致死的。可利用的存活至蛹期的Chriz亚型具有正常的染色体表型。减少Z4蛋白不会影响Chriz与多线染色体的结合，反之亦然。当Chriz蛋白通过RNA干扰被耗尽时，Z4仍然是染色体结合的。
Polytene interphase chromosomes are compacted into a series of bands and interbands reflecting their organization into independent chromosomal domains. In order to understand chromosomal organization, we set out to study the role of proteins that are selective for interbands. Here we describe the Drosophila melanogaster chromodomain protein Chriz that is coimmunoprecipitated with the zinc finger protein Z4. Both proteins colocalize exclusively to the interbands on Drosophila polytene chromosomes. Like Z4, Chriz is ubiquitously expressed throughout development and is associated with chromatin in all interphase nuclei. Following dissociation from chromatin, early in mitosis Chriz binds to the centrosomes and to the mitotic spindle. Newly induced amorphic Chriz alleles are early lethal, and ubiquitous overexpression of Chriz is lethal as well. Available Chriz hypomorphs which survive until pupal stage have a normal chromosomal phenotype. Reducing Z4 protein does not affect Chriz binding to polytene chromosomes and vice versa. Z4 is still chromosomally bound when Chriz protein is depleted by RNA interference.