Cytochrome P450 1A-Dependent Activities in Deer, Cattle and Horses
Cytochrome P450 1A-Dependent Activities in Deer, Cattle and Horses
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DOI：
10.1292/jvms.09-0318
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发表时间：
2010-05-01
影响因子：
1.2
通讯作者：
Ishizuka, Mayumi
中科院分区：
文献类型：
--
作者：
Darwish, Wageh Sobhy;Ikenaka, Yoshinori;Ishizuka, Mayumi
本研究的目的是用乙氧基间苯二酚O-脱乙基化法(EROD)和甲氧基间苯二酚O-脱甲基(MROD)法比较研究CYP1A在鹿、牛和马体内的代谢活性，并与大鼠的代谢活性进行比较。我们使用抗大鼠细胞色素P1A1抗体对这些活性进行了抑制研究，并确定这些活性是由于细胞色素P1A亚家族。在这项研究中，依赖于细胞色素P1a的活性存在高度的种间差异。特别是，我们发现在所研究的动物物种中，马的EROD和MROD活性最高。在动力学分析中，马的Vmax和催化效率(Vmax/Km)最高，其次是牛、鹿和鼠。
The objective of this study was to investigate and characterize the metabolic activities of CYP1A in deer, cattle and horses in comparison to those of rats using ethoxyresorufin O-deethylation (EROD) and methoxyresorufin O-demethylation (MROD) assays. We performed an inhibition study for these activities using anti-rat CYP1A1 antibody and identified that these activities were due to the CYP1A subfamily. Interspecies differences in the CYP1A-dependent activities were highly observed in this study. In particular, we found that the horse had the highest EROD and MROD activities among the examined animal species. In the kinetic analysis, the horses showed the highest Vmax and catalytic efficiency (Vmax/Km), followed by the cattle, deer and rats.