Two novel blaNDM-1 harboring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food-animal source Enterobacteriaceae.
Two novel blaNDM-1 harboring transposons on pPrY2001-like plasmids coexisting with a novel cfr-encoding plasmid in food-animal source Enterobacteriaceae.
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DOI：
10.1016/j.jgar.2021.06.006
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发表时间：
2021-07
影响因子：
4.6
通讯作者：
Xing-Run Zheng;Yin-Huan Sun;Jia-Hang Zhu;Si-Li Wu;Caiyan Ping;Liang-xing Fang;Hong-Xia Jiang
中科院分区：
文献类型：
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作者：
Xing-Run Zheng;Yin-Huan Sun;Jia-Hang Zhu;Si-Li Wu;Caiyan Ping;Liang-xing Fang;Hong-Xia Jiang
目的本研究报告从食用动物的碳青霉烯类耐药肠杆菌中鉴定出碳青霉烯酶编码基因。方法通过 PCR 检测从一个农场的 475 个粪便拭子中回收的总共 40 个细菌分离株是否存在 blaNDM-1 基因。通过 MALDI-TOF/MS 对 ThreeblaNDM-1 阳性菌株进行物种鉴定。通过微量肉汤稀释法进行抗菌药物敏感性测试。通过过滤器配对测定blaNDM-1和cfr基因的可转移性。通过全基因组测序分析blaNDM-1和cfr的遗传环境。结果2只奇异变形杆菌(JPM24和YPM35)和1只雷氏普罗维登斯菌(YPR25)携带blaNDM-1。 blaNDM-1 基因位于可接合的 pPrY2001 样质粒上，据报道通常携带重要的抗菌素耐药基因 (ARG)。 YPR25 和 YPM35 共享两个几乎相同的可接合质粒，一个携带 blaNDM-1，另一个携带 cfr。 YPR25（与YPM35相同）和JPM24中的blaNDM-1基因位于两个新的转座子中，分别命名为Tn6922和Tn6923。 Tn6922和Tn6923分别携带14个和7个ARG，均含有同一方向的多个IS26拷贝，具有高度相似性。此外，cfr 位于 YPR25 中未报告的高接合转移频率的质粒上（与 YPM35 相同）。结论我们鉴定了 pPrY2001 样质粒上存在的两个含有新颖blaNDM-1 的转座子（Tn6922 和 Tn6923）。 pPrY2001-likeblaNDM-1 质粒与新颖的 cfr 质粒共存，并且两者都可以高频率转移，凸显了连续监测可作为 ARG 储存库的动物源性多重耐药肠杆菌的重要性。
ObjectivesThis study reports identification of the carbapenemase-encoding gene from carbapenem-resistant Enterobacterales from food animals.MethodsA total of 40 bacterial isolates recovered from 475 faecal swabs obtained on one farm were tested for the presence of theblaNDM-1gene by PCR. Species identification of threeblaNDM-1-positive strains was conducted by MALDI-TOF/MS. Antimicrobial susceptibility testing was performed by broth microdilution. Transferability of theblaNDM-1andcfrgenes was determined by filter mating. The genetic environment ofblaNDM-1andcfrwas analysed by whole-genome sequencing.ResultsTwoProteus mirabilis(JPM24 and YPM35) and oneProvidencia rettgeri(YPR25) carriedblaNDM-1. TheblaNDM-1genes were located on conjugable pPrY2001-like plasmids often reported to carry important antimicrobial resistance genes (ARGs). YPR25 and YPM35 shared two almost identical conjugable plasmids, one carryingblaNDM-1and the othercfr. TheblaNDM-1gene in YPR25 (same as YPM35) and JPM24 was located in two novel transposons, designated Tn6922and Tn6923, respectively. Tn6922and Tn6923carried 14 and 7 ARGs, respectively, and both contained multiple copies of IS26in the same direction, with a high degree of similarity. Additionally,cfrwas located on a plasmid with an unreported high frequency of conjugative transfer in YPR25 (same as YPM35).ConclusionWe identified two novelblaNDM-1-containing transposons (Tn6922and Tn6923) present on pPrY2001-like plasmids. The pPrY2001-likeblaNDM-1plasmids coexisted with a novelcfrplasmid, and both could transfer at high frequency, highlighting the importance of continuous surveillance of multiresistant Enterobacterales of animal origin that can serve as a reservoir for ARGs.