A numbers game: Mosquito-based arbovirus surveillance in two distinct geographic regions of Latin America.
A numbers game: Mosquito-based arbovirus surveillance in two distinct geographic regions of Latin America.
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数字游戏：在拉丁美洲两个不同地理区域进行基于蚊子的虫媒病毒监测。
DOI：
10.1101/2024.03.15.585246
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Cevallos,Varsovia
中科院分区：
文献类型：
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作者：
Mojica,Jacqueline;Arévalo,Valentina;Juarez,JoseG;Galarza,Ximena;Gonzalez,Karla;Carrazco,Andrés;Suazo,Harold;Harris,Eva;Coloma,Josefina;Ponce,Patricio;Balmaseda,Angel;Cevallos,Varsovia
伊蚊作为医学上重要的节肢动物传播病毒（虫媒病毒）的载体，构成了重大的公共卫生威胁，需要昆虫学和流行病学监测来指导病媒控制计划，以预防和减少疾病传播。在这项研究中，我们展示了在拉丁美洲两个不同地理区域（尼加拉瓜和厄瓜多尔）进行的为期 1 年的埃及伊蚊（Linnaeus，1762）基于蚊子的虫媒病毒监测的合作成果。使用背包吸引器在来自 8 个不同社区（尼加拉瓜：2 个，厄瓜多尔：6 个）的 2,800 多个随机选择的家庭（尼加拉瓜、厄瓜多尔）和 100 个关键地点（尼加拉瓜）收集成年雌性蚊子。使用实时逆转录聚合酶链式反应对总共 1,358 个雌性蚊子库进行了 RNA 提取和病毒 RNA 检测。检测到 10 个阳性登革热病毒 (DENV) 库（尼加拉瓜 3 个，厄瓜多尔 7 个），所有这些都是在雨季发现的，并且与人类中发现的血清型相匹配（尼加拉瓜：DENV-1 和 DENV-4；厄瓜多尔：DENV-2）。感染率从1.13到23.13不等，其中尼加拉瓜社区的感染率最低。我们的结果证明了在资源匮乏的环境中检测感染登革热病毒的伊蚊的可行性，并强调了有针对性的蚊子虫媒病毒采样和测试的必要性，为拉丁美洲地区未来的监测计划提供了宝贵的见解。
Aedesmosquitoes, as vectors of medically important arthropod-borne viruses (arboviruses), constitute a major public health threat that requires entomological and epidemiological surveillance to guide vector control programs to prevent and reduce disease transmission. In this study, we present the collaborative effort of 1 year ofAedes ageypti(Linnaeus, 1762) mosquito-based arbovirus surveillance in 2 geographically distinct regions of Latin America (Nicaragua and Ecuador). Adult female mosquitoes were collected using backpack aspirators in over 2,800 randomly selected households (Nicaragua, Ecuador) and 100 key sites (Nicaragua) from 8 distinct communities (Nicaragua: 2, Ecuador: 6). A total of 1,358 mosquito female pools were processed for RNA extraction and viral RNA detection using real-time reverse transcription-polymerase chain reaction. Ten positive dengue virus (DENV) pools were detected (3 in Nicaragua and 7 in Ecuador), all of which were found during the rainy season and matched the serotypes found in humans (Nicaragua: DENV-1 and DENV-4; Ecuador: DENV-2). Infection rates ranged from 1.13 to 23.13, with the Nicaraguan communities having the lowest infection rates. Our results demonstrate the feasibility of detecting DENV-infectedAedesmosquitoes in low-resource settings and underscore the need for targeted mosquito arbovirus sampling and testing, providing valuable insights for future surveillance programs in the Latin American region.