Side-effect test of sorafenib using 3-D skin equivalent based on microfluidic skin-on-a-chip
Side-effect test of sorafenib using 3-D skin equivalent based on microfluidic skin-on-a-chip
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DOI：
10.1016/j.jiec.2019.09.044
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发表时间：
2020-02-25
影响因子：
6.1
通讯作者：
Sung, Gun Yong
中科院分区：
文献类型：
--
作者：
Jeon, Hye Mi;Kim, Kyunghee;Sung, Gun Yong
索拉非尼是一种新生血管阻断剂，通过靶向血管生成的多种激酶抑制剂来防止新血管的形成，从而防止癌组织的生长。然而，索拉非尼的患者报告了几种副作用。因此，本研究调查索拉非尼的皮肤副作用所揭示的苏木精和伊红（H&E）染色，实时聚合酶链反应（RT-PCR），免疫组化染色结果使用人皮肤等效模型产生的人成纤维细胞和角质形成细胞。观察到颗粒的增厚和通过颗粒层上的变化表达的蛋白质，类似于实际患者的组织。预计本研究的结果将大大改善临床前评价，并促进皮肤芯片系统在药物副作用和化妆品功效评价中的应用。(C)2019年韩国工业和工程化学学会。Elsevier B. V.出版，保留所有权利。
Sorafenib is a neovascular blocker that prevents the formation of new blood vessels, and by consequence, the growth of cancer tissue, through multiple kinase inhibitors that target angiogenesis. However, several side effects have been reported by patients on sorafenib. Therefore, this study investigates the skin side effects of sorafenib as revealed by hematoxylin and eosin (H&E) staining, real-time polymerase chain reaction (RT-PCR), and immunohistochemical staining results using a human skin equivalent model produced from human fibroblast and keratinocyte. Thickening of the granules and the protein expressed through the changes on the granular layer, similar to the tissues of an actual patient, was observed. It is expected that the findings of this study will greatly improve the preclinical evaluations and promote the use of the skin-on-a-chip system for evaluating the side effects of drugs and the efficacy of cosmetics. (C) 2019 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.