New experimental model for adoptive transfer of murine autoimmune orchitis
New experimental model for adoptive transfer of murine autoimmune orchitis
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小鼠自身免疫性睾丸炎过继转移新实验模型
作者：
Masahiro Itoh;A. Mukasa;Y. Tokunaga;C. Hiramine;K. Hojo
摘要实验性自身免疫性睾丸炎（experimental autoimmune orchitis，EAO）是C3 H/He小鼠经皮下注射（s.c.）注射活的同基因睾丸生殖细胞（TC），而不求助于任何佐剂或免疫增强剂。利用该EAO模型，开发了一种新的简单的EAO过继转移方案。细胞供体是接受s.c.注射两次单独TC。在体外用TC刺激来自供体的脾细胞，在含白细胞介素-2的培养基中增殖，然后腹膜内注射给未处理的受体小鼠。该手术引起严重的睾丸炎和精子生成不足，有或没有炎症的附睾和输精管在高发病率的受体。在转移前清除所有T细胞或CD 4 + T细胞在受体中不产生组织病理学体征，而清除CD 8 + T细胞或B细胞对疾病转移没有抑制作用，表明效应细胞是CD 4 + T细胞。
Summary. Previous studies demonstrated that experimental autoimmune orchitis (EAO) was produced in C3H/He mice with very high incidence by subcutaneous (s.c.) injections of viable syngeneic testicular germ cells (TC), without resorting to any adjuvants or immunopotentiators. Using this EAO model, a new and simple protocol was developed for adoptive transfer of EAO. Cell donors were C3H/He mice that received s.c. injections twice with TC alone. Spleen cells from the donors were stimulated in vitro with TC, propagated in interleukin‐2 containing medium, then injected i.p. to naive recipient mice. This procedure induced severe orchitis and hypospermatogenesis with or without inflammation in epididymis and vas deferens in the recipients at high incidence. Elimination of all T cells or CD4+ T cells before the transfer produced no histopathological signs in the recipients whereas that of the CD8+ T cells or B cells had no inhibitory effect on the disease transfer, indicating that the effector cells are CD4+ T cells.