Identification of serum microRNAs as diagnostic and prognostic biomarkers for acute pancreatitis
Identification of serum microRNAs as diagnostic and prognostic biomarkers for acute pancreatitis
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鉴定血清 microRNA 作为急性胰腺炎的诊断和预后生物标志物
DOI：
10.1016/j.pan.2014.03.019
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发表时间：
2014-05-01
期刊：
影响因子：
3.6
通讯作者：
Lv, Nong-hua
中科院分区：
文献类型：
--
作者：
Liu, Pi;Xia, Liang;Lv, Nong-hua
背景/目的：鉴定血清microRNA （miRNA）作为急性胰腺炎（AP）诊断和预后的生物标志物。材料和方法：分析了12例不同疾病严重程度的AP患者和3名健康对照者的血清microRNA表达。差异表达的mirna在更大的患者和对照组队列中得到验证。采用受试者工作特征（ROC）曲线分析评估差异表达的miRNA的诊断和预后潜力，并与AP的经典预后标志物进行比较。结果：miRNA微阵列分析在AP患者和对照组血清中鉴定出205种差异表达的miRNA。9种mirna在重度和轻度AP患者中表达差异。进一步的验证证实了AP患者中miR-92b、miR-10a和miR-7的下调，ROC分析显示这些mirna可以区分AP与健康病例。此外，在疾病并发症或低血浆钙水平的患者中，血清miR-551b-5p水平显著升高。ROC分析显示，血清miR-551b-5p水平可区分重度和轻度AP。结论：miR-92b、miR-10a、miR-7在AP中的表达可用于AP的早期诊断，miR-551b-5p可用于预测AP的严重程度。版权所有：IAP和EPC。由爱思唯尔印度出版，爱思唯尔印度有限公司的一个部门。版权所有。
Background/objectives: To identify serum microRNA (miRNA) as diagnostic and prognostic biomarkers for acute pancreatitis (AP).Materials and methods: Sera microRNA expression was profiled from 12 AP patients with varying disease severity and three healthy controls. Differentially expressed miRNAs were validated in a larger cohort of patients and controls. The diagnostic and prognostic potentials of differentially expressed miRNAs were evaluated using receiver operating characteristic (ROC) curve analysis and compared to that of classic prognostic markers for AP.Results: miRNA microarray analyses identified 205 differentially expressed miRNAs between sera from AP patients and that from controls. Nine miRNAs were differentially expressed between severe and mild AP patients. Further validation confirmed the down-regulation of miR-92b, miR-10a, and miR-7 in AP patients, and ROC analysis revealed that these miRNAs can differentiate AP from health cases. Furthermore, the serum miR-551b-5p level was significantly higher in patients with disease complications or a low plasma calcium level. ROC analysis showed that the serum miR-551b-5p level can distinguish between severe and mild AP.Conclusion: The expressions of miR-92b, miR-10a, and miR-7 in AP might be used for the early diagnosis of AP and miR-551b-5p may be used for predicting AP severity. Copyright (C) 2014, IAP and EPC. Published by Elsevier India, a division of Reed Elsevier India Pvt. Ltd. All rights reserved.