LIGHT-STIMULATED PROTEIN MOVEMENT IN ROD PHOTORECEPTOR CELLS OF THE RAT RETINA
LIGHT-STIMULATED PROTEIN MOVEMENT IN ROD PHOTORECEPTOR CELLS OF THE RAT RETINA
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DOI：
10.1016/0014-5793(87)81144-4
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发表时间：
1987-12-10
期刊：
影响因子：
3.5
通讯作者：
LONG, K
中科院分区：
文献类型：
--
作者：
PHILP, NJ;CHANG, W;LONG, K
我们检查了参与视觉传导的环GMP级联的三种蛋白质的细胞内分布; cGMP磷酸二酯酶，G蛋白的α亚基和抑制蛋白。在成年大鼠中，通过纯化ROS的聚丙烯酰胺凝胶分析和视网膜切片上的免疫细胞化学定位，观察到光诱导的感光细胞外节中G和抑制蛋白量的变化。在黑暗条件下，G主要集中在感光细胞的外节，而在光照条件下，Gα主要集中在内节和外核层。Arrestin的分布与此相反，在黑暗条件下出现在内节和外核层，在光照条件下出现在ROS中。相比之下，PDE，由G激活并由arrestin抑制的酶，没有显示出光刺激的运动。在光适应和暗适应的视网膜中，PDE主要位于感光细胞的外段。
We examined the intracellular distribution of three proteins involved in the cyclic GMP cascade of visual transduction; cGMP phosphodiesterase, the α‐subunit of G‐protein and arrestin. In adult rats, light‐induced changes in the amounts of G and arrestin in the photoreceptor cell outer segments were observed both by polyacrylamide gel analysis of purified ROS and by immunocytochemical localization on retinal sections. In dark conditions, G was concentrated in the outer segments of photoreceptor cells while in the light Gαwas seen in the inner segments and the outer nuclear layer. Arrestin had the opposite distribution, appearing in the inner segments and outer nuclear layer under dark conditions and in the ROS under light conditions. In contrast, PDE, the enzyme which is activated by G and inhibited by arrestin showed no light‐stimulated movement. In both light‐ and dark‐adapted retinas, PDE was localized primarily in the outer segments of the photoreceptor cells.