Effects of single and repeated administrations of Toki-shakuyaku-san on the concentrations of brain neurotransmitters in mice
Effects of single and repeated administrations of Toki-shakuyaku-san on the concentrations of brain neurotransmitters in mice
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DOI：
10.1358/mf.1998.20.1.485617
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发表时间：
1998-01-01
影响因子：
--
通讯作者：
Masuda, Y
中科院分区：
文献类型：
--
作者：
Itoh, T;Murai, S;Masuda, Y
采用高效液相色谱-电子捕获检测法（HPLC-ECD）测定了小鼠经口给予Toki-shakuyoku-san（TSS）粉末提取物后大脑皮层、纹状体和海马中单胺相关物质（NE、MHPG、DA、DOPAC、HVA、5-HT和5-HIAA）和乙酰胆碱（ACh）的含量。单次给予TSS 50 mg/kg可降低海马NE含量，增加DA、DOPAC和HVA含量。TSS 500 mg/kg单次给药可增加大脑皮质DA、HVA含量，增加海马DA、DOPAC、HVA含量，降低NE含量。TSS 50 mg/kg重复给药（2次/d，共14 d）可增加大脑皮层DA、DOPAC和5-HIAA的含量，500 mg/kg可增加大脑皮层NE、MHPG、DOPAC、5-HT和5-HIAA的含量，增加纹状体NE和DA的含量。对于乙酰胆碱含量，单次和重复给药TSS在50和500毫克/公斤没有影响，在小鼠脑的三个区域。结果提示，TSS单次给药可刺激小鼠海马多巴胺能神经系统的功能，抑制肾上腺素能神经系统的功能，而TSS重复给药可刺激大脑皮质肾上腺素能、多巴胺能和肾上腺素能神经系统的功能。TSS对小鼠脑ACh含量无影响。
The amounts of monoamine-related substances (NE, MHPG, DA, DOPAC, HVA, 5-HT and 5-HIAA) and acetylcholine (ACh) in the cerebral cortex, corpus striatum and hippocampus of mice treated orally with a powdered extract of Toki-shakuyoku-san (TSS) were measured using the HPLC-ECD method. Single administration of TSS at 50 mg/kg decreased the content of NE in the hippocampus, but increased the contents of DA, DOPAC and HVA. Single administration of TSS at 500 mg/kg increased the contents of DA and HVA in the cerebral cortex and those of DA, DOPAC and HVA in the hippocampus, but decreased the content of NE. Repeated administration (twice a day, for 14 days) of TSS at 50 mg/kg increased the contents of DA, DOPAC and 5-HIAA in the cerebral cortex, while 500 mg/kg increased the contents of NE, MHPG, DOPAC, 5-HT and 5-HIAA in the cerebral cortex, and the NE and DA in the corpus striatum. With regard to ACh content, single and repeated administrations of TSS at 50 and 500 mg/kg had no influence in the three regions of mouse brain. These results suggest that single administration of TSS stimulates the function of the dopaminergic nervous system in the hippocampus in mice and inhibits the function of the adrenergic nervous system, and that repeated administration of TSS stimulates the function of the adrenergic, dopaminergic and serotonergic nervous systems in th cerebral cortex. TSS, however, did no show any influence on the brain ACh content in mice.