Uridine addition after microRNA-directed cleavage
Uridine addition after microRNA-directed cleavage
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DOI：
10.1126/science.1103521
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发表时间：
2004-11-05
期刊：
影响因子：
56.9
通讯作者：
Goodman, HM
中科院分区：
文献类型：
--
作者：
Shen, BZ;Goodman, HM
One of the important roles of microRNA (miRNA) is to direct the cleavage of messenger RNA (mRNA). However, the mechanisms of decay of the cleaved mRNA products is not well understood. We show that miRNA-directed cleavage products in organisms as diverse asArabidopsis, mouse, and Epstein-Barr virus have at their 3' ends a stretch (1 to 24 nucleotides) of oligouridine posttranscriptionally added downstream of the cleavage site. This 3' uridine addition, as shown forArabidopsis, is correlated with decapping and 5' shortening of the cleaved products, suggesting a mechanistic step in the miRNA-directed mRNA decay mechanism.
