Sialoglycoconjugate expression in primary colorectal cancer and metastatic lymph node tissues.
Sialoglycoconjugate expression in primary colorectal cancer and metastatic lymph node tissues.
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发表时间：
2007
影响因子：
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通讯作者：
Y. Inagaki;Wei Tang;Q. Guo;N. Kokudo;Y. Sugawara;Hirona Karako;T. Konishi;M. Nakata;H. Nagawa;M. Makuuchi
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文献类型：
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作者：
Y. Inagaki;Wei Tang;Q. Guo;N. Kokudo;Y. Sugawara;Hirona Karako;T. Konishi;M. Nakata;H. Nagawa;M. Makuuchi
背景/目的细胞表面异常糖基化，尤其是过度唾液酸化，被认为在肿瘤恶性肿瘤中具有重要意义。为了探讨结直肠癌唾液酸化的临床病理学意义，我们用唾液酸结合凝集素进行了组织化学分析。方法采用凝集素-免疫组化方法，对80例大肠癌及淋巴结组织进行了MAL和SNA染色。统计分析其染色特征与各种临床病理参数之间的关系。结果原发癌组织中SNA高表达与预后不良及淋巴结转移等病理特征相关，而MAL高表达与预后不良相关。在转移淋巴结组织中，MAL和SNA常呈阳性表达，在N2转移组中阳性率最高。此外，转移淋巴结组织中MAL阳性表达的病例明显比MAL阴性表达的病例预后差。结论原发性结直肠癌组织中SNA阳性唾液酸糖缀合物的异常表达是结直肠癌不良病理特征的重要因素。此外，MAL阳性唾液酸糖缀合物在转移淋巴结组织中的异常表达可能与较差的预后有关。
BACKGROUND/AIMS Aberrant cell surface glycosylation, and especially excessive sialylation, is thought to have great importance in tumor malignancy. To investigate the clinicopathological significance of sialylation in colorectal cancer, we performed histochemical analyses using sialic acid-binding lectins. METHODOLOGY Primary colorectal cancer and lymph node tissues obtained from 80 cases were subjected to lectin-immunohistochemical staining using Maackia amurensis leukoagglutinin (MAL) and Sambucus nigra agglutinin (SNA). The relationship between the staining characteristics and the various clinicopathological parameters was statistically analyzed. RESULTS In primary cancer tissues, a high level of SNA staining was significantly related to worse prognosis and some pathological characteristics such as lymph node metastasis, whereas a high level of MAL staining was related to worse prognosis. In metastatic lymph node tissues, positive staining was frequently found for MAL and SNA, which wasremarkable in cases categorized as N2 metastasis. Furthermore, cases with MAL-positive staining in metastatic lymph node tissues evidently indicated worse prognosis than those with MAL-negative staining. CONCLUSIONS Aberrant expression of SNA-positive sialoglycoconjugates in primary colorectal cancer tissues is important in terms of unfavorable pathological characteristics of the cancer. In addition, aberrant expression of MAL-positive sialoglycoconjugates in metastatic lymph node tissues might be related to worse prognosis.