Molecular imaging of endogenous and exogenous chromophores using ground state recovery pump-probe optical coherence tomography
Molecular imaging of endogenous and exogenous chromophores using ground state recovery pump-probe optical coherence tomography
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DOI：
10.1364/oe.14.009142
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发表时间：
2006-10-02
期刊：
影响因子：
3.8
通讯作者：
Izatt, Joseph A.
中科院分区：
文献类型：
--
作者：
Applegate, Brian E.;Izatt, Joseph A.
我们提出了一种新的分子成像技术，它结合了三维层析成像能力的光学相干断层扫描与泵浦探测光谱的分子灵敏度。这种基于瞬时吸收的技术对任何分子发色团都很敏感。这是特别有前途的许多重要的生物标志物，如血红蛋白，这是穷人的荧光团，因此难以与目前的光学技术没有化学标记的图像。泵浦-探测光学相干层析成像的先前实现遭受低效的泵浦-探测方案，这损害了该技术的灵敏度和适用性。在这里，我们通过避免稳态动力学和自发过程，有利于测量瞬态吸收的完全允许的电子跃迁在很短的时间尺度之前，达到稳态利用过去的泵探测方法的效率进行优化。在这篇文章中，我们详细介绍了原型系统的优化和表征，比较实验结果的系统灵敏度的理论预测。我们展示了两种不同发色团的组织样本的原位成像;可转染蛋白dsRed和蛋白血红蛋白。我们还证明，与一个简单的样品容器和人全血和罗丹明6 G的混合物，潜在的使用基态恢复时间分离的贡献多个发色团的基态恢复信号。(c)2006年美国光学学会
We present a novel molecular imaging technique which combines the 3-D tomographic imaging capability of optical coherence tomography with the molecular sensitivity of pump-probe spectroscopy. This technique, based on transient absorption, is sensitive to any molecular chromophore. It is particularly promising for the many important biomarkers, such as hemoglobin, which are poor fluorophores and therefore difficult to image with current optical techniques without chemical labeling. Previous implementations of pump-probe optical coherence tomography have suffered from inefficient pump-probe schemes which hurt the sensitivity and applicability of the technique. Here we optimize the efficiency of the pump-probe approach by avoiding the steady-state kinetics and spontaneous processes exploited in the past in favor of measuring the transient absorption of fully allowed electronic transitions on very short time scales before a steady-state is achieved. In this article, we detail the optimization and characterization of the prototype system, comparing experimental results for the system sensitivity to theoretical predictions. We demonstrate in situ imaging of tissue samples with two different chromophores; the transfectable protein dsRed and the protein hemoglobin. We also demonstrate, with a simple sample vessel and a mixture of human whole blood and rhodamine 6G, the potential to use ground state recovery time to separate the contributions of multiple chromophores to the ground state recovery signal. (c) 2006 Optical Society of America