Super-resolved 3D-STED microscopy identifies a layer-specific increase in excitatory synapses in the hippocampal CA1 region of Neuroligin-3 KO mice
Super-resolved 3D-STED microscopy identifies a layer-specific increase in excitatory synapses in the hippocampal CA1 region of Neuroligin-3 KO mice
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超分辨率 3D-STED 显微镜发现 Neuroligin-3 KO 小鼠海马 CA1 区域兴奋性突触的层特异性增加
DOI：
10.1016/j.bbrc.2021.10.003
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发表时间：
2021
影响因子：
3.1
通讯作者：
Kawabe Hiroshi
中科院分区：
文献类型：
--
作者：
Koganezawa Noriko;Hanamura Kenji;Schwark Manuela;Krueger-Burg Dilja;Kawabe Hiroshi
化学突触是一种细胞粘附系统，它将信息从大脑复杂神经元网络中的一个神经元传递到另一个神经元。突触传递是神经网络信息处理过程中的限速步骤，其可塑性与认知功能有关。因此，突触的形态学和电生理学分析在神经科学研究中特别重要。在当前的研究中，我们应用超分辨率三维受激发射损耗（3D-STED）显微镜进行突触的形态分析。这种方法使我们能够估计小鼠海马组织中兴奋性和抑制性突触的精确数量。我们用这种方法发现了自闭症谱系障碍模型小鼠 Neuroligin-3KO 中兴奋性突触的区域特异性增加。这种类型的分析将在未来开辟发育神经科学的新领域。
The chemical synapse is one type of cell-adhesion system that transmits information from a neuron to another neuron in the complex neuronal network in the brain. Synaptic transmission is the rate-limiting step during the information processing in the neuronal network and its plasticity is involved in cognitive functions. Thus, morphological and electrophysiological analyses of synapses are of particular importance in neuroscience research. In the current study, we applied super-resolved three-dimensional stimulated emission depletion (3D-STED) microscopy for the morphological analyses of synapses. This approach allowed us to estimate the precise number of excitatory and inhibitory synapses in the mouse hippocampal tissue. We discovered a region-specific increase in excitatory synapses in a model mouse of autism spectrum disorder,Neuroligin-3KO, with this method. This type of analysis will open a new field in developmental neuroscience in the future.