Channel crossing: how are proteins shipped across the bacterial plasma membrane?
Channel crossing: how are proteins shipped across the bacterial plasma membrane?
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DOI：
10.1098/rstb.2015.0025
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发表时间：
2015-10-05
期刊：
影响因子：
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通讯作者：
Allen WJ
中科院分区：
文献类型：
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作者：
Collinson I;Corey RA;Allen WJ
第一个蛋白质传导通道的结构是在十多年前确定的。蛋白质转运是蛋白质跨膜和跨膜转运的动力学机制，至今仍困扰着我们。这篇综述试图总结和理解蛋白质转运机器的能量转导能力，重点是细菌系统：多肽如何对脂质双层取得进展，以及该过程如何与ATP水解和跨膜蛋白动力相关的自由能耦合。为了探索货物是如何被驱动穿过膜的，蛋白质易位机器的已知结构以历史文献为背景，并根据在它们之后进行的实验来阐述。本文将集中在细菌的一般分泌（Sec）途径（SecY复合物），其真核生物的对应物（Sec61复合物），渡轮蛋白质跨膜在未折叠状态，以及无关的达特系统，组装定制的通道出口折叠的蛋白质。
The structure of the first protein-conducting channel was determined more than a decade ago. Today, we are still puzzled by the outstanding problem of protein translocation—the dynamic mechanism underlying the consignment of proteins across and into membranes. This review is an attempt to summarize and understand the energy transducing capabilities of protein-translocating machines, with emphasis on bacterial systems: how polypeptides make headway against the lipid bilayer and how the process is coupled to the free energy associated with ATP hydrolysis and the transmembrane protein motive force. In order to explore how cargo is driven across the membrane, the known structures of the protein-translocation machines are set out against the background of the historic literature, and in the light of experiments conducted in their wake. The paper will focus on the bacterial general secretory (Sec) pathway (SecY-complex), and its eukaryotic counterpart (Sec61-complex), which ferry proteins across the membrane in an unfolded state, as well as the unrelated Tat system that assembles bespoke channels for the export of folded proteins.