Evaluation of CD4+ T cell function In vivo in HIV-infected patients as measured by bacteriophage phiX174 immunization.
Evaluation of CD4+ T cell function In vivo in HIV-infected patients as measured by bacteriophage phiX174 immunization.
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DOI：
10.1086/315739
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发表时间：
2000-08
期刊：
影响因子：
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通讯作者：
Ilana Fogelman;V. Davey;Hans D. Ochs-Hans D.-Ochs-2254298500;Michael Elashoff;M. B. Feinberg;J. Mican;Jay P. Siegel-Jay P.-Siegel-2254001668;Michael Sneller;H. Lane
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文献类型：
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作者：
Ilana Fogelman;V. Davey;Hans D. Ochs-Hans D.-Ochs-2254298500;Michael Elashoff;M. B. Feinberg;J. Mican;Jay P. Siegel-Jay P.-Siegel-2254001668;Michael Sneller;H. Lane
噬菌体phiX174免疫被用来检测体内人类免疫缺陷病毒(HIV)感染患者体内所有疾病阶段的CD4(+)T细胞功能。通过测量T细胞在抗体产生、扩增和同型转换方面为B细胞提供帮助的能力来评估功能。33名患者和10名对照共接种了3次噬菌体phiX174疫苗，间隔6周。患者对噬菌体特异性总抗体滴度和免疫球蛋白滴度的反应在数量和质量上都低于对照组的反应。总体而言，33名患者中有7名T细胞功能正常。基线CD4计数与总抗体和免疫球蛋白滴度的相关性最强。HIV RNA与应答的相关性较弱，但在CD4计数为200个/微升的患者中具有一定的预测能力。噬菌体phiX174免疫似乎是检测体内免疫功能的有用工具，这表明尽管接受了足够的抗逆转录病毒治疗，但大多数HIV感染者可能存在CD4(+)T细胞功能异常。
Bacteriophage phiX174 immunization was used to measure CD4(+) T cell function in vivo in human immunodeficiency virus (HIV)-infected patients across all disease stages. Function was evaluated by measuring the ability of T cells to provide help to B cells in antibody production, amplification, and isotype switching. A total of 33 patients and 10 controls received 3 bacteriophage phiX174 immunizations 6 weeks apart. The patients' responses regarding bacteriophage-specific total antibody titers and IgG titers were quantitatively and qualitatively inferior to the controls' responses. Overall, 7 of 33 patients had normal T cell function. Baseline CD4 counts provided the strongest correlation with total antibody and IgG titers. HIV RNA had a weaker association with responses but had some predictive power among patients with a CD4 count >200 cells/microL. Bacteriophage phiX174 immunization seems to be a useful tool for measuring immune function in vivo, which suggests that most HIV-infected patients may have abnormal CD4(+) T cell function despite adequate antiretroviral treatment.