Chromatin techniques for plant cells
Chromatin techniques for plant cells
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DOI：
10.1111/j.1365-313x.2004.02169.x
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发表时间：
2004-09-01
期刊：
影响因子：
7.2
通讯作者：
Paszkowski, J
中科院分区：
文献类型：
--
作者：
Bowler, C;Benvenuto, G;Paszkowski, J
最近的大量研究表明，植物发育的许多重要方面都是由基因表达的可遗传变化调节的，而不涉及DNA序列的变化。相反，这些调控机制涉及染色质结构的改变，这些结构影响靶基因对能够控制其表达的调控因子的可及性。染色质的中心成分是核小体，它含有高度保守的组蛋白蛋白，已知这些蛋白会受到广泛的翻译后修饰，这些蛋白是染色质相关因子结合的识别密码。除了这些组蛋白修饰，DNA甲基化也可以对基因表达产生巨大影响。为了适应植物科学界对植物发育表观遗传控制的日益增长的兴趣，我们的实验室已经汇编了一系列常规使用的方法，这些方法可以在生化、分子和细胞水平上促进假定的染色质结合因子的表征。
A large number of recent studies have demonstrated that many important aspects of plant development are regulated by heritable changes in gene expression that do not involve changes in DNA sequence. Rather, these regulatory mechanisms involve modifications of chromatin structure that affect the accessibility of target genes to regulatory factors that can control their expression. The central component of chromatin is the nucleosome, containing the highly conserved histone proteins that are known to be subject to a wide range of post-translational modifications, which act as recognition codes for the binding of chromatin-associated factors. In addition to these histone modifications, DNA methylation can also have a dramatic influence on gene expression. To accommodate the burgeoning interest of the plant science community in the epigenetic control of plant development, a series of methods used routinely in our laboratories have been compiled that can facilitate the characterization of putative chromatin-binding factors at the biochemical, molecular and cellular levels.