MDR: an integrative DNA N6-methyladenine and N4-methylcytosine modification database for Rosaceae
MDR: an integrative DNA N6-methyladenine and N4-methylcytosine modification database for Rosaceae
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MDR：蔷薇科 DNA N6-甲基腺嘌呤和 N4-甲基胞嘧啶综合修饰数据库
DOI：
10.1038/s41438-019-0160-4
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发表时间：
2019-06-15
影响因子：
8.7
通讯作者：
Xiao, Chuan-Le
中科院分区：
文献类型：
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作者：
Liu, Zhao-Yu;Xing, Jian-Feng;Xiao, Chuan-Le
真核生物DNA甲基化因其重要的表观遗传调控功能而受到越来越多的关注。最近开发的单分子实时（SMRT）测序技术提供了一种有效的方法来检测DNA N6-甲基腺嘌呤（6 mA）和N4-甲基胞嘧啶（4 mC）修饰在单核苷酸分辨率。蔷薇科包含具有广泛经济重要性的园艺植物。然而，目前知之甚少的全基因组分布模式和功能的6 mA和4 mC修饰的蔷薇科。在这项研究中，我们提出了一个完整的DNA 6 mA和4 mC修饰数据库蔷薇科（MDR，http：//mdr. xieslab。org）。MDR是第一个用于显示和存储来自蔷薇科SMRT测序数据集的DNA 6 mA和4 mC甲基化组的存储库，包括Meta和统计信息、甲基化密度、基因本体富集分析以及NCBI中甲基化位点的基因组搜索和浏览。MDR提供了关于DNA 6 mA和4 mC甲基化的重要信息，可以帮助用户更好地了解蔷薇科中的表观遗传修饰。
Eukaryotic DNA methylation has been receiving increasing attention for its crucial epigenetic regulatory function. The recently developed single-molecule real-time (SMRT) sequencing technology provides an efficient way to detect DNA N6-methyladenine (6mA) and N4-methylcytosine (4mC) modifications at a single-nucleotide resolution. The family Rosaceae contains horticultural plants with a wide range of economic importance. However, little is currently known regarding the genome-wide distribution patterns and functions of 6mA and 4mC modifications in the Rosaceae. In this study, we present an integrated DNA 6mA and 4mC modification database for the Rosaceae (MDR, http://mdr. xieslab. org). MDR, the first repository for displaying and storing DNA 6mA and 4mC methylomes from SMRT sequencing data sets for Rosaceae, includes meta and statistical information, methylation densities, Gene Ontology enrichment analyses, and genome search and browse for methylated sites in NCBI. MDR provides important information regarding DNA 6mA and 4mC methylation and may help users better understand epigenetic modifications in the family Rosaceae.