DNA polymerase delta holoenzyme: action on single-stranded DNA and on double-stranded DNA in the presence of replicative DNA helicases.
DNA polymerase delta holoenzyme: action on single-stranded DNA and on double-stranded DNA in the presence of replicative DNA helicases.
复制标题
DNA 聚合酶 δ 全酶：在复制 DNA 解旋酶存在的情况下作用于单链 DNA 和双链 DNA。
DOI：
10.1021/bi00015a011
复制
发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Hübscher,U
中科院分区：
文献类型：
--
作者：
Podust,VN;Podust,LM;Müller,F;Hübscher,U
Revised Manuscript Received January 17, 1995® abstract: DNA polymerase d requires proliferating cell nuclear antigen and replication factor C to form a holoenzyme efficient in DNA synthesis. We have analyzed three different aspects of calf thymus DNA polymerase 6 holoenzyme:(i) analysis of pausing during DNA synthesis,(ii) replication of double-stranded DNA in the absence of additional factors, and (iii) replication of double-stranded DNA in the presence of the two known replicative DNA helicases from simian virus 40 and bovine papilloma virus.DNA polymerase (5 holoenzyme replicated primed single-stranded DNA at a rate of 100—300 nucleotides/min, partially overcoming multiple pause sites on DNA. WhileEscherichia coli single-strand DNA binding protein helped DNA polymerase< 5 pass through pause sites, the DNA polymerase 6 itself appeared to dissociate from the template in the absence of synthesis or when encountering pausesites. Proliferating cell nuclear antigen likely remained on the template. DNA polymerase d holoenzyme could perform limited strand displacement synthesis on double-stranded gapped circular DNA, and this reaction was not stimulated either by replication protein A or by E. coli single-strand DNA binding protein. DNA polymerase d holoenzyme could efficiently cooperate with replicative DNA helicases from simian virus 40 (large T antigen) and bovine papilloma virus 1(protein El) in replication through double-stranded DNA in a reaction that required replication protein A or E. coli single-strand DNA bindingprotein. Our data are consistent with the role of DNA polymerase d as the leading strand replicasebut also suggest that additional factors [eg, proteins to pass pause sites and cellular DNA helicase (s)] might be required to achieve replication at a physiological speed.
