Evaluation of Erythrocyte Changes After Normoxic Return from Hypoxia.
Evaluation of Erythrocyte Changes After Normoxic Return from Hypoxia.
复制标题
从缺氧恢复常氧后红细胞变化的评估。
DOI：
10.1007/978-1-4939-7665-2_16
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Prchal,JosefT
中科院分区：
文献类型：
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作者：
Song,Jihyun;Prchal,JosefT
低氧通过低氧诱导因子（HIF），主要是通过HIF-2，其上调促红细胞生成素转录，增加红细胞生成。这导致红细胞（RBC）产量增加，并向组织输送更多的氧气。在迅速恢复至常氧状态后，缺氧诱导的红细胞增多症被新细胞溶解过度纠正，新细胞溶解是由来自膨胀线粒体的活性氧物质（ROS）引起的优先携带低过氧化氢酶（由缺氧刺激的microRNA（miR）-21下调）的年轻RBC的短暂破坏。为了研究新生红细胞溶解的分子机制，区分年轻和老年红细胞的寿命和检测缺氧处理前后血液学的变化是至关重要的。在这里，我们描述这些测量的方法方面。
Hypoxia increases erythropoiesis by hypoxia-inducible factors (HIF), principally by HIF-2, which upregulates erythropoietin transcription. This results in an increase of red blood cell (RBC) production and delivery of more oxygen to tissues. Upon rapid return to normoxia, hypoxia-induced polycythemia is overcorrected by neocytolysis, a transient destruction of preferentially young RBCs bearing low catalase (downregulated by hypoxia-stimulated microRNA(miR)-21) caused by reactive oxygen species (ROS) from expanded mitochondria. In order to study molecular mechanism of neocytolysis, it is critical to differentiate life span of young and old RBCs and to measure the hematological changes before and after hypoxia treatment. Here we describe the methodological aspects of these measurements.