Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice.
Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice.
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DOI：
10.1126/science.2847315
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发表时间：
1988-11
期刊：
影响因子：
56.9
通讯作者：
David W. Tank;M. Sugimori;John A. Connor;R. R. Llinás-R.
中科院分区：
文献类型：
--
作者：
David W. Tank;M. Sugimori;John A. Connor;R. R. Llinás-R.
应用荧光显微成像技术研究了豚鼠小脑浦肯野细胞内钙离子浓度与电压依赖性电活动的关系。细胞内钙离子浓度的时空动态过程中表现出自发和诱发活动。这一结果与电生理实验所提出的钙电导的枝晶偏析假说相一致。这些体外切片荧光成像方法适用于中枢神经系统生化和电生理功能的广泛问题。
Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.