Thyroxine 5'-deiodinase activity in brown adipose tissue.
Thyroxine 5'-deiodinase activity in brown adipose tissue.
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DOI：
10.1210/endo-112-3-1153
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发表时间：
1983-03
期刊：
影响因子：
4.8
通讯作者：
Jack L. Leonard;Sarah A. Mellen;Reed P. Larsen
中科院分区：
文献类型：
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作者：
Jack L. Leonard;Sarah A. Mellen;Reed P. Larsen
大鼠棕色脂肪组织（BAT）和附睾脂肪垫（EPI）的匀浆在二硫苏糖醇存在下催化3，3 '，5'-三碘甲状腺原氨酸（rT 3）的5 '-脱碘。BAT（而非EPI）催化甲状腺素E（T4）的5 '-脱碘，产生等量的3，5，3'-三碘甲状腺原氨酸（T3）和碘化物。BAT 5 '-脱碘酶活性不受丙基硫氧嘧啶（PTU）的影响，在甲状腺功能减退大鼠中升高，而EPI 5'-脱碘酶完全被PTU抑制，在甲状腺功能减退大鼠中被抑制。这些结果表明，BAT中存在II型（PTU不敏感）5 '-碘甲状腺原氨酸脱碘酶活性。
Homogenates of rat brown adipose tissue (BAT) and epididymal fat pad (EPI) catalyze the 5'-deiodination of 3,3',5'-triiodothyronine (rT3) in the presence of dithiothreitol. BAT, but not EPI, catalyzes the 5'-deiodination of thyroxinE (T4) generating equal amounts of 3,5,3'-triiodothyronine (T3) and iodide. BAT 5'-deiodinase activity was unaffected by propylthiouracil (PTU) and was elevated in hypothyroid rats, whereas EPI 5'-deiodinase was totally inhibited by PTU and was depressed in hypothyroidism. These results indicate that type II (PTU-insensitive) 5'-iodothyronine deiodinase activity is present in BAT.