Role of siderophore in Pseudomonas fluorescens biofilm formation and spoilage potential function
Role of siderophore in Pseudomonas fluorescens biofilm formation and spoilage potential function
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DOI：
10.1016/j.fm.2022.104151
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发表时间：
2022-09-29
影响因子：
5.3
通讯作者：
Wang，Yanbo
中科院分区：
文献类型：
--
作者：
Chen，Jian;Sun，Zhihua;Wang，Yanbo
我们研究了绿脓菌荧光素在荧光假单胞菌生物膜形成、运动性和腐败潜力中的功能。我们靶向并鉴定了参与铁载体生物合成的两个主要基因（pvdA和pvdE）。然后我们构建了P.荧光标记PF 08，并发现绿脓菌荧光素的缺失导致生物膜到动力的转变，因为Δ pvdA和Δ pvdE突变体都显示出增强的运动性、降低的胞外多糖（EPS）水平和减弱的生物膜形成。此外，缺乏绿脓菌荧光素的合成促进了在4 °C下储存的鱼片的腐败。基于绿脓菌荧光素缺失对表型的影响，我们报道了绿脓菌荧光素调节htpX、phoA、flip、flgA和RpoS的转录水平，表明绿脓菌荧光素介导的铁吸收可能影响鞭毛和胁迫抗性的调节。这项研究强调了绿脓菌荧光素在生物膜的形成、运动和腐败中的重要作用。荧光PF 08。
We investigated the function of pyoverdine in the biofilm formation, motility, and spoilage potential of Pseudomonas fluorescens. We targeted and identified two major genes (pvdAandpvdE) that are involved in the biosynthesis of siderophores. We next constructed ΔpvdAand ΔpvdEmutants ofP. fluorescensPF08 and found that the deletion of pyoverdine led to a biofilm-to-motivity transition as both ΔpvdAand ΔpvdEmutants displayed enhanced motility, reduced level of exopolysaccharides (EPSs), and attenuated biofilm formation. In addition, the lack of synthesis of pyoverdine promoted the spoilage of fish fillets stored at 4 °C. Based on the effect of pyoverdine deletion on the phenotype; we report that pyoverdine regulates the transcription levels ofhtpX,phoA,flip,flgA,andRpoS, suggesting that pyoverdine-mediated iron absorption may affect the regulation of flagellum and stress resistance. This study emphasizes the important role of pyoverdine in the formation of biofilm, motility, and spoilage ofP. fluorescensPF08.