Nitration of chemokine CXCL8 acts as a natural mechanism to limit acute inflammation.
Nitration of chemokine CXCL8 acts as a natural mechanism to limit acute inflammation.
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趋化因子CXCL8的硝化作用是限制急性炎症的天然机制。
DOI：
10.1007/s00018-022-04663-x
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发表时间：
2023-01-09
影响因子：
8
通讯作者：
Ali, Simi
中科院分区：
文献类型：
--
作者：
Thompson, Sarah;Pang, Chong Yun;Sepuru, Krishna Mohan;Cambier, Seppe;Hellyer, Thomas P.;Scott, Jonathan;Simpson, A. John;Proost, Paul;Kirby, John A.;Rajarathnam, Krishna;Sheerin, Neil S.;Ali, Simi
Chemokine CXCL8 is a key facilitator of the human host immune response, mediating neutrophil migration, and activation at the site of infection and injury. The oxidative burst is an important effector mechanism which leads to the generation of reactive nitrogen species (RNS), including peroxynitrite. The current study was performed to determine the potential for nitration to alter the biological properties of CXCL8 and its detection in human disease. Here, we show peroxynitrite nitrates CXCL8 and thereby regulates neutrophil migration and activation. The nitrated chemokine was unable to induce transendothelial neutrophil migration in vitro and failed to promote leukocyte recruitment in vivo. This reduced activity is due to impairment in both G protein-coupled receptor signaling and glycosaminoglycan binding. Using a novel antibody, nitrated CXCL8 was detected in bronchoalveolar lavage samples from patients with pneumonia. These findings were validated by mass spectrometry. Our results provide the first direct evidence of chemokine nitration in human pathophysiology and suggest a natural mechanism that limits acute inflammation. The online version contains supplementary material available at 10.1007/s00018-022-04663-x.
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影响因子：
6
作者：
Lux, Moritz;Blaut, Alexander;Vielhauer, Volker
通讯作者：
Vielhauer, Volker
影响因子：
8
作者：
Joseph, Prem Raj B.;Rajarathnam, Krishna
通讯作者：
Rajarathnam, Krishna
影响因子：
6.4
作者：
Martinez-Burgo, Beatriz;Cobb, Steven L.;Ali, Simi
通讯作者：
Ali, Simi
影响因子：
8
作者：
Cambier S;Metzemaekers M;de Carvalho AC;Nooyens A;Jacobs C;Vanderbeke L;Malengier-Devlies B;Gouwy M;Heylen E;Meersseman P;Hermans G;Wauters E;Wilmer A;CONTAGIOUS Consortium;Schols D;Matthys P;Opdenakker G;Marques RE;Wauters J;Vandooren J;Proost P
通讯作者：
Proost P
DOI：
10.1186/1710-1492-2-3-98
发表时间：
2006-09-15
期刊：
Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology
影响因子：
--
作者：
Lacy P
通讯作者：
Lacy P