Olfactory-enriched transcripts are cell-specific markers in the lobster olfactory organ
Olfactory-enriched transcripts are cell-specific markers in the lobster olfactory organ
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DOI：
10.1002/cne.10489
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发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
McClintock, TS
中科院分区：
文献类型：
--
作者：
Hollins, B;Hardin, D;McClintock, TS
在组织中特异性表达的基因通常参与该组织的定义功能。我们使用代表性差异分析的cDNA扩增20个cDNA片段，代表更丰富的龙虾嗅觉器官比大脑，眼睛/眼柄，趾，pereiopod，或第二天线的成绩单。然后，我们独立地证实了这些克隆所代表的转录本在嗅觉器官中富集。20个cDNA片段代表6至15个不同的基因。其中6个cDNA含有与已知基因家族高度相似的序列。我们进行了原位杂交与这六个，发现所有的表达在与嗅觉器官的感觉器相关的细胞亚群。克隆OET-07（一种离子型受体）和OET-10（一种α微管蛋白）对嗅觉受体神经元具有特异性。OET-02是一种单加氧酶，仅在外辅助细胞中表达。OET-03是一种丝氨酸蛋白酶，对领细胞具有特异性。OET-11是一种α 2巨球蛋白，由受体神经元和领细胞表达。OET-17是一种钙磷肽，在受体神经元、内辅助细胞和领细胞中表达。这六个转录本的身份和表达模式预测参与龙虾嗅觉器官的已知和新特性。(C)2002 Wiley-Liss，Inc.
Genes expressed specifically in a tissue are often involved in the defining functions of that tissue. We used representational difference analysis of cDNA to amplify 20 cDNA fragments representing transcripts that were more abundant in the lobster olfactory organ than in brain, eye/eyestalk, dactyl, pereiopod, or second antenna. We then independently confirmed that the transcripts represented by these clones were enriched in the olfactory organ. The 20 cDNA fragments represent between 6 and 15 different genes. Six of the cDNAs contained sequences highly similar to known gene families. We performed in situ hybridization with these six and found that all were expressed in subsets of cells associated with the aesthetasc sensilla in the olfactory organ. Clones OET-07, an ionotropic receptor, and OET-10, an alpha tubulin, were specific to the olfactory receptor neurons. OET-02, a monooxygenase, was expressed only in the outer auxiliary cells. OET-03, a serine protease, was specific to the collar cells. OET-11, an alpha(2) macroglobulin, was expressed by the receptor neurons and the collar cells. OET-17, a calcyphosine, was expressed in the receptor neurons, inner auxiliary cells, and collar cells. The identities and expression patterns of these six transcripts predict involvement in both known and novel properties of the lobster olfactory organ. (C) 2002 Wiley-Liss, Inc.