Early Alterations in Cytokine Expression in Adult Compared to Developing Lung in Mice after Radiation Exposure
Early Alterations in Cytokine Expression in Adult Compared to Developing Lung in Mice after Radiation Exposure
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DOI：
10.1667/rr1882.1
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发表时间：
2010-04-01
影响因子：
3.4
通讯作者：
Williams, Jacqueline P.
中科院分区：
文献类型：
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作者：
Johnston, Carl J.;Hernady, Eric;Williams, Jacqueline P.
为了评估低剂量辐射暴露后肺部的早期变化（可能作为恐怖事件后减轻肺损伤的目标），我们分析了辐射后细胞因子的表达。对成年小鼠进行全肺或全身照射后的研究。全身照射后还对不同年龄的幼鼠进行了研究。分析组织和血浆中的 mRNA 丰度，并评估病理变化。在肺组织中，照射后 1 小时和 6 小时，IL1B、IL1R2 和 CXCR2 mRNA 表达出现剂量相关变化，同时血浆 KC/CXCL1 和 IL6 蛋白水平增加。然而，在幼崽中，直到 28 天龄（与出生后肺部生长结束一致）才检测到 IL1 丰度的变化，尽管在所有年龄段都检测到细胞凋亡。总之，尽管细胞因子在低剂量辐射后表达，但它们在组织反应进展中的作用仍有待确定。它们可能是用于基于标记的生物剂量测定的候选者。然而，生命早期缺乏细胞因子诱导表明儿童可能需要不同的终点（和缓解治疗）。 (C) 2010 年放射线研究会
To assess early changes in the lung after low-dose radiation exposure that may serve as targets for mitigation of lung injury in the aftermath of a terrorist event, we analyzed cytokine expression after irradiation. Adult mice were studied after whole-lung or total-body irradiation. Mouse pups of different ages were also investigated after total-body irradiation. mRNA abundance was analyzed in tissue and plasma, and pathological changes were assessed. In lung tissue, dose-related changes were seen in IL1B, IL1R2 and CXCR2 mRNA expression at 1 and 6 h after irradiation, concurrent with increases in plasma protein levels of KC/CXCL1 and IL6. However, in the pups, changes in IL1 abundance were not detected until 28 days of age, coincident with the end of postnatal lung growth, although apoptosis was detected at all ages. In conclusion, although cytokines were expressed after low doses of radiation, their role in the progression of tissue response is yet to be determined. They may be candidates for use in marker-based biodosimetry. However, the lack of cytokine induction in early life suggests that different end points (and mitigating treatments) may be required for children. (C) 2010 by Radiation Research Society