Molecular and biological characterization of Cowpea mild mottle virus isolates infecting soybean in Brazil and evidence of recombination
Molecular and biological characterization of Cowpea mild mottle virus isolates infecting soybean in Brazil and evidence of recombination
复制标题
感染巴西大豆的豇豆轻度斑驳病毒分离株的分子和生物学特征及重组证据
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
C. M. Carvalho
中科院分区：
文献类型：
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作者：
L. G. Zanardo;F. N. Silva;A. C. Bicalho;Gloria Patricia Castillo Urquiza;A. Lima;Á. Almeida;F. Zerbini;C. M. Carvalho
报道了6株豇豆轻度斑疹病毒（CPMMV; carlavvirus, Betaflexiviridae）新毒株的生物学和分子特性。在巴西巴伊亚州、戈亚斯州、马托格罗索州和米纳斯吉拉斯州收集到花叶病和茎坏死的大豆植株。CPMMV分离株的完整基因组长8180-8198个核苷酸（nt），不包括3 ' -聚腺苷化的尾部，并且与CPMMV的加纳分离株具有67-68% nt序列一致性，这是唯一一个基因组测序的CPMMV分离株。该复制酶仅与加纳CPMMV分离物具有60 - 61%的序列同源性，其外壳蛋白（CP）高度保守（79%的序列同源性和95-96%的氨基酸序列同源性）。高CP一致性和系统发育分析支持巴西分离株为CPMMV的分类。发现了与加纳pmmv分离株的生物学和分子差异，并表明这6株分离株代表一种独特的CPMMV菌株，命名为CPMMV- br。此外，研究表明重组主要发生在聚合酶基因上，而在CPMMV基因组的其他区域可能较少发生。关键词：卡拉病毒，CPMMV，系统发育，重组
The biological and molecular characterization of six isolates of a new Cowpea mild mottle virus strain (CPMMV;Carlavirus, Betaflexiviridae) are reported. Soybean plants with mosaic and stem necrosis were collected in Bahia, Goias,Mato Grosso and Minas Gerais states, Brazil. Complete genomes of the CPMMV isolates are 8180–8198 nucleotides(nt) long, excluding the 3′-polyadenylated tail, and have 67–68% nt sequence identity with a Ghana isolate ofCPMMV, the only CPMMV isolate for which the genome has previously been sequenced. The replicase has only60–61% nt sequence identity with the Ghana CPMMV isolate, and the coat protein (CP) is highly conserved (79% ntsequence identity and 95–96% amino acid sequence identity). The high CP identity and the phylogenetic analysessupported the classification of the Brazilian isolates as CPMMV. Biological and molecular differences with the GhanaCPMMV isolate were found and indicated that the six isolates represent a distinct CPMMV strain denominated asCPMMV-BR. Furthermore, it is shown that recombination occurred mainly in the polymerase gene, and may occur lessfrequently in other regions of the CPMMV genome.Keywords: Carlavirus, CPMMV, phylogeny, recombination