Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species
Simultaneous visualization of multiple antigens with tyramide signal amplification using antibodies from the same species
复制标题
DOI：
10.1369/jhc.6a7134.2007
复制
发表时间：
2007-06-01
影响因子：
3.2
通讯作者：
Mezey, Eva
中科院分区：
文献类型：
--
作者：
Toth, Zsuzsanna E.;Mezey, Eva
在免疫组织化学 (IHC) 开始常规使用后，许多研究人员致力于研究在同一组织切片或细胞中对多个分子进行染色的方法。 Achieving this goal was not easy, however.原因之一是 IHC 反应中使用的大多数一抗都是在兔子中产生的，识别来自两种不同兔子抗体的信号并非易事。因此，迄今为止描述的所有协议都具有严重的局限性。在这里，我们报告了一个简单、快速且廉价的解决方案。与现有方法相比，它有两个主要优点。首先，通过使用来自同一宿主的抗体，可以使用市售荧光染料在同一切片中显现两种或多种抗原。其次，由于该技术依赖于信号放大，因此可以检测稀有和丰富的抗原。
After immunohistochemistry (IHC) began to be used routinely, a number of investigators worked on methods for staining multiple molecules in the same tissue sections or cells. Achieving this goal was not easy, however. One reason for this is that the majority of primary antibodies used in IHC reactions are raised in rabbits, and recognizing signals from two different rabbit antibodies is not trivial. Thus, all of the protocols described to date have serious limitations. Here we report a simple, quick, and inexpensive solution to the problem. it has two major advantages over existing methods. First, by using antibodies from the same host, two or more antigens can be visualized in the same section with commercially available fluorescent dyes. Second, because the technique relies on signal amplification, both rare and abundant antigens can be detected.