Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells
Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells
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DOI：
10.1271/bbb.70.980
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发表时间：
2006-04-01
影响因子：
1.6
通讯作者：
Maeshima, M
中科院分区：
文献类型：
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作者：
Kobae, Y;Mizutani, M;Maeshima, M
水通道蛋白介导水在生物膜上的运动。拟南芥含有35个水通道蛋白，分别属于PIP、TIP、SIP和NIP四个亚家族。我们在悬浮培养的拟南芥细胞中研究了它们在生长过程中以及对盐和渗透胁迫的反应的免疫化学表达谱。所有水通道蛋白的蛋白量在培养细胞中比在植物组织中要低得多。这与培养细胞原生质体的低透水性是一致的。NaCl处理后，PIP2蛋白量；1、PIP2;2、PIP2；细胞中3的表达增加数倍，TIP1的表达增加数倍；1和TIPI；分别是2倍、15倍和3倍。PIP1在压力下没有变化。细胞在培养19 d后开始死亡，伴随着PIPs和TIPs的明显积累，SIPs逐渐下降。我们的研究结果表明：(i)水通道蛋白异构体的积累在单个细胞中受到低水平的单独调节。至少PIP2；2、PIP2;3, T1P1;1、TIP1；其中2种是悬浮细胞中的应激反应型水通道蛋白。（iii） PIP2和TIP1亚家族成员的突然增加可能与细胞死亡有关。
Aquaporins mediate the movement of water across biomembranes. Arabidopsis thaliana contains 35 aquaporins that belong to four subfamilies (PIP, TIP, SIP, and NIP). We investigated their expression profiles immunochemically in suspension-cultured Arabidopsis thaliana cells during growth and in response to salt and osmotic stresses. Protein amounts of all aquaporins were much lower in cultured cells than in the plant tissues. This is consistent with the low water permeability of protoplasts from cultured cells. After treatment with NaCl, the protein amounts of PIP2;1, PIP2;2, and PIP2;3 in the cells increased several-fold, and those of TIP1;1 and TIPI;2,15- and 3-fold respectively. PIP1 did not change under the stress. Cell death began after 19 d in culture, accompanied by marked accumulation of PIPs and TIPs and a gradual decrease in SIPs. Our results suggest the followings: (i) Accumulation of aquaporin isoforms was individually regulated at low levels in single cells. (ii) At least PIP2;2, PIP2;3, T1P1;1, and TIP1;2 are stress-responsive aquaporins in suspension cells. (iii) A sudden increment of several members of PIP2 and TIP1 subfamilies might be related to cell death.