Effects of L-arginine on aggregates of fatty-acid/potassium soap in the aqueous media
Effects of L-arginine on aggregates of fatty-acid/potassium soap in the aqueous media
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DOI：
10.1007/s00396-005-1423-1
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发表时间：
2006-02-01
影响因子：
2.4
通讯作者：
Maeda, H
中科院分区：
文献类型：
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作者：
Hirai, A;Kawasaki, H;Maeda, H
在本研究中，我们研究了L-精氨酸对脂肪酸/脂肪皂在水介质中聚集体的影响，作为pH值的函数，通过氢离子滴定，粘弹性测量，冷冻透射电子显微镜和相差显微镜。我们发现L-精氨酸有效地抑制油酸或辛酸的油滴生长。该效应被解释为精氨酸在微观液滴表面的吸附，或通过脂肪酸的羧基和精氨酸的羧酸盐之间的氢键辅助的疏水效应在油/水微界面。至于月桂酸在低于烃链熔点的温度下的结晶，精氨酸是无效的。此外，我们还发现精氨酸阳离子与油酸阴离子胶束的强结合诱导了优势胶束的生长。L-精氨酸已被用于许多重折叠系统中以抑制蛋白质聚集。L-精氨酸在本研究中观察到的水介质中脂肪酸/脂肪皂聚集体的这些作用预计将形成蛋白质重折叠中显示的特定功能的基础。
In the present study, we investigated the effects of L-arginine on aggregates of fatty acid/fatty soap in the aqueous media as a function of pH, by means of hydrogen ion titration, viscoelastic measurement, cryotransmission electron microscopy and phase contrast microscopy. We found out that L-arginine effectively inhibits the oil droplet growth of oleic acid or octanoic acid. The effect is explained in terms of the adsorption of arginine at the microscopic drop surface, or at the oil/water microinterface through the hydrophobic effect assisted by the hydrogen bonds between carboxyl group of fatty acid and carboxylate of arginine. As to the crystallization of lauric acid at temperatures below the melting point of the hydrocarbon chain, arginine is not effective. In addition, we also found out that the strong binding of arginine cation to anionic oleate micells induces the dominant micellar growth. L-arginine has been used in many refolding systems to suppress protein aggregation. These effects of L-arginine on the aggregates of fatty acid/fatty soap in the aqueous media observed in the present study is expected to form a basis to the specific function displayed in the protein refolding.