Effects of copper and its ethylenediaminetetraacetate complex on the antioxidant defenses of the goldfish, Carassius auratus.
Effects of copper and its ethylenediaminetetraacetate complex on the antioxidant defenses of the goldfish, Carassius auratus.
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DOI：
10.1016/j.ecoenv.2005.09.002
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发表时间：
2006-11
影响因子：
6.8
通讯作者：
Hui Liu;Weimu Wang;Jingfei Zhang;Xiaorong Wang
中科院分区：
文献类型：
--
作者：
Hui Liu;Weimu Wang;Jingfei Zhang;Xiaorong Wang
在金鱼的组织中的铜的生物利用度和抗氧化剂的防御在鱼的肝脏中进行了研究，在体内暴露于不同种类的铜溶液在不同的浓度40天后。铜似乎是更集中在鳃比在肝脏后Cu 2+和铜-乙二胺四乙酸（Cu-EDTA）曝光。添加EDTA对鳃中铜的吸附有显著影响，但对肝脏无显著影响。在低于国家渔业水质标准（0.01mg/L）的Cu ~（2+）暴露40 d后，肝脏超氧化物歧化酶（SOD）和谷胱甘肽S-转移酶（GST）活性升高，过氧化氢酶（CAT）和硒依赖的谷胱甘肽过氧化物酶（Se-GPx）活性降低。与铜暴露相比，EDTA对SOD和GST的影响不显著。而CAT和GPx活性在Cu ~（2+）处理下与Cu ~（2+）-EDTA处理下差异显著。
Copper bioavailability in the tissues of goldfish and antioxidant defenses in the liver of fish were investigated in vivo following 40 days of exposure to different species of copper solutions at different concentrations. Copper seemed to be more concentrated in the gill than in the liver after Cu2+and copper-ethylenediaminetetraacetate (Cu–EDTA) exposure. The addition of EDTA markedly affected copper adsorption in the gill but had no significant effects in the liver. We also found that in the fish exposed to the concentration of Cu2+below the Chinese national fishery water quality standard of 0.01mg/L, superoxide dismutase (SOD) and glutathione S-transferase (GST) activities in liver were stimulated and catalase (CAT) and Se-dependent glutathione peroxidase (Se-GPx) activities were inhibited after 40 days of exposure. Compared to copper exposure, the addition of EDTA did not affect SOD and GST significantly. However, CAT and GPx activity with Cu2+exposure were significantly different from those with Cu–EDTA exposure.