Biodegradable microspheres made of conductive polyorganophosphazene showing antioxidant capacity for improved bone regeneration
Biodegradable microspheres made of conductive polyorganophosphazene showing antioxidant capacity for improved bone regeneration
复制标题
由导电聚有机磷腈制成的可生物降解微球具有抗氧化能力，可改善骨再生
DOI：
10.1016/j.cej.2020.125352
复制
发表时间：
2020-10-01
影响因子：
15.1
通讯作者：
Yang, Xiaoping
中科院分区：
文献类型：
--
作者：
Huang, Yiqian;Du, Zhiyun;Yang, Xiaoping
活性氧（ROS）可能在严重骨缺损周围积聚，从而危害周围细胞的活性并阻碍新骨形成。解决这一问题的有效策略是开发具有抗氧化和骨诱导能力的支架生物材料。合成了一种苯胺四聚体（AT）和甘氨酸乙酯共取代的聚有机磷腈（PATGP），由于AT部分具有抗氧化性，而富磷腈部分具有骨相容性，有望满足要求。此外，AT赋予PATGP导电性以匹配骨组织的电生理。采用体外细胞培养和体内评价方法，以聚丙氨酰乙基甘氨酸磷腈（PAGP）和聚丙交酯-乙交酯共聚物（PLGA）微球为对照，对PATGP微球型支架的ROS清除能力、细胞毒性和骨诱导活性进行了系统的研究。其中，PATGP微球表现出最强的促进作用，上调细胞活性和加速新生骨形成大鼠颅骨缺损。与聚酯型生物材料相比，总之，聚有机磷腈在功能化方面表现出很强的灵活性，通过引入补充功能，如抗氧化活性和电活性，这使得它们在增强骨生成方面非常有效。
Reactive oxygen species (ROS) are likely to accumulate around severe bone defects, which jeopardizes activities of surrounding cells and hampers new bone formation. An effective strategy to address this issue is to develop scaffolding biomaterials with both antioxidant and osteoinductive capacities. An aniline tetramer (AT) and glycine ethyl ester co-substituted polyorganophosphazene (PATGP) was synthesized, and expected to meet the demands, since the AT moieties were antioxidant and the phosphorus-rich phosphazene moieties were osteocompatible. Moreover, the AT endowed the PATGP with conductivity to match the electrophysiology of bone tissues. By applying in vitro cell culture and in vivo evaluations, microsphere-type scaffolds made of PATGP were systematically characterized on their capacities including ROS-scavenging effect, cytotoxicity and osteoinductivity, using non-conductive poly[(ethylalanato)(ethylglycinato)]phosphazene (PAGP) and poly(lactide-co-glycolide) (PLGA) microspheres as control groups. Among them, PATGP microspheres demonstrated the strongest promotion effects on up-regulating cellular activities and on speeding up neobone formation in rat calvarial defects. Compared to polyester-type biomaterials, in summary, polyorganophosphazenes demonstrated strong flexibility in functionalization by introducing supplementary features such as antioxidant activity and electroactivity, which made them to be quite efficient in enhancing osteogenesis.