Cell cycle-related bystander responses are not increased with LET after heavy-ion irradiation
Cell cycle-related bystander responses are not increased with LET after heavy-ion irradiation
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DOI：
10.1667/rr0760.1
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发表时间：
2007-02-01
影响因子：
3.4
通讯作者：
Taucher-Scholz, G.
中科院分区：
文献类型：
--
作者：
Fournier, C.;Becker, D.;Taucher-Scholz, G.
越来越多的证据表明，受辐射的细胞会影响其未受辐射的邻居，因此它们反过来会表现出通常与直接辐射暴露相关的细胞反应。这些反应通常被称为旁观者效应。在这项研究中，研究了三种人类成纤维细胞在暴露于高密度电离辐射后与细胞周期相关的旁观者反应。通过将线性能量转移 (LET) 从 11 变化到 15,000 keV mu m(-1)，可以研究诱导细胞中 DNA 损伤的复杂性对旁观细胞反应的影响。使用宽光束和微光束照射，获得了诱导 CDKN1A 的瞬时旁观者反应 (p21)。当旁观者信号的传输仅限于可溶性因素时，也观察到后者。对汇合细胞单层中的单个细胞进行靶向照射显示，旁观者细胞中 CDKN1A 蛋白的量与目标细胞的径向距离之间没有相关性。与不同 LET 照射后旁观者细胞中 CDKN1A 的诱导一致，观察到 G(0)/G(1) 细胞照射后第一个 G 期的短暂延迟。然而，CDKN1A 诱导显示对过早终末分化没有显着影响，过早终末分化被认为是受辐射组织纤维化的基础。因此，旁观者细胞中未改变的分化模式并不表明明显的、持久的影响。 (c) 2007 年，辐射研究协会。
Evidence has accumulated that irradiated cells affect their unirradiated neighbors, so that they in turn display cellular responses typically associated with direct radiation exposure. These responses are generally known as bystander effects. In this study, cell cycle-related bystander responses were investigated in three strains of human fibroblasts after exposure to densely ionizing radiation. Varying the linear energy transfer (LET) from 11 to 15,000 keV mu m(-1) allowed a study of the impact of the complexity of DNA damage in the inducing cells on the responses of bystander cells. Using both broad-beam and microbeam irradiation, transient bystander responses were obtained for the induction of CDKN1A (p21). The latter was also observed when the transmission of bystander signals was limited to soluble factors. Targeted irradiation of single cells in confluent cell monolayers revealed no correlation between the amount of CDKN1A protein in the bystander cells and the radial distance to the targeted cells. In line with the induction of CDKN1A in bystander cells after irradiation with different LETs, a transient delay in the first G, phase after irradiation of G(0)/G(1) cells was observed. However, the CDKN1A induction revealed no significant effect on premature terminal differentiation considered to underlie fibrosis in irradiated tissue. Thus the unchanged differentiation pattern in bystander cells does not indicate pronounced, long-lasting effects. (c) 2007 by Radiation Research Society.