Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation
Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation
复制标题
Osf2/Cbfa1：成骨细胞分化的转录激活因子
DOI：
10.1016/s0092-8674(00)80257-3
复制
发表时间：
1997-05-30
期刊：
影响因子：
64.5
通讯作者：
Karsenty, G
中科院分区：
文献类型：
--
作者：
Ducy, P;Zhang, R;Karsenty, G
成骨细胞是骨形成细胞。成骨细胞特异性基因表达和分化的分子基础尚不清楚。我们先前在骨钙素启动子中发现了一种成骨细胞特异性顺式作用元件，称为OSE2。我们现在已经克隆了编码OSE2结合蛋白Osf2/Cbfa1的cDNA。Osf2/Cbfa1的表达始于发育中骨骼的间质凝聚，此后严格限制在成骨细胞系的细胞中，并受BMP7和维生素D-3的调节。Osf2/Cbfa1结合并调节成骨细胞中表达的多种基因的表达。最后，在非成骨细胞中强制表达Osf2/Cbfa1可以诱导主要成骨细胞特异性基因的表达。本研究确定Osf2/Cbfa1是成骨细胞特异性转录因子，是成骨细胞分化的调节因子。
The osteoblast is the bone-forming cell. The molecular basis of osteoblast-specific gene expression and differentiation is unknown. We previously identified an osteoblast-specific cis-acting element, termed OSE2, in the Osteocalcin promoter. We have now cloned the cDNA encoding Osf2/Cbfa1, the protein that binds to OSE2. Osf2/Cbfa1 expression is initiated in the mesenchymal condensations of the developing skeleton, is strictly restricted to cells of the osteoblast lineage thereafter, and is regulated by BMP7 and vitamin D-3. Osf2/Cbfa1 binds to and regulates the expression of multiple genes expressed in osteoblasts. Finally, forced expression of Osf2/Cbfa1 in nonosteoblastic cells induces the expression of the principal osteoblast-specific genes. this study identifies Osf2/Cbfa1 as an osteoblast-specific transcription factor and as a regulator of osteoblast differentiation.