Alteration in cellular functions in mouse macrophages after exposure to 50 Hz magnetic fields
Alteration in cellular functions in mouse macrophages after exposure to 50 Hz magnetic fields
复制标题
DOI：
10.1002/jcb.20920
复制
发表时间：
2006-09-01
影响因子：
4
通讯作者：
Simko, Myrtill
中科院分区：
文献类型：
--
作者：
Frahm, Jana;Lantow, Magareta;Simko, Myrtill
本研究旨在探讨极低频电磁场（ELF-EMF）对小鼠巨噬细胞某些细胞功能和免疫学参数的影响。在这项研究中，在1.0 mT的50 Hz磁场（MF）的影响进行了研究对吞噬活性和分化的巨噬细胞的白细胞介素-1 β（IL-1 β）的生产。MF暴露导致45分钟后增加的吞噬活性，显示为与对照相比，MF暴露的细胞中乳胶珠的摄取增加1.6倍。我们还证明了24小时暴露（1.0 mT MF）后巨噬细胞中IL-1 β释放增加。与对照组相比，时间依赖性IL-1 β形成在4小时后已显著增加，并在24小时后达到最大值12.3倍。本研究的另一方面是通过分析长期（12、24和48 h）暴露的巨噬细胞中的微核（MN）形成来检查1.0 mT MF的遗传毒性能力。我们的数据显示，暴露细胞的MN形成或不规则的有丝分裂活动没有显着差异。此外，测试了50 Hz MF的不同通量密度（范围从0.05到1.0 mT，持续45 min）对自由基形成的影响，作为小鼠巨噬细胞前体细胞中细胞活化的终点。所有测试的通量密度显着刺激自由基的形成。在这里，我们证明了极低频电磁场刺激小鼠巨噬细胞的生理细胞功能的能力显着升高的吞噬活性，自由基释放，和IL-1 β的生产表明细胞活化能力的极低频电磁场在没有任何遗传毒性作用。
The aim of the present study is to investigate whether extremely low frequency electromagnetic fields (ELF-EMF) affect certain cellular functions and immunologic parameters of mouse macrophages. In this study, the influence of 50 Hz magnetic fields (MF) at 1.0 mT was investigated on the phagocytic activity and on the interleukin-1 beta (IL-1 beta ) production in differentiated macrophages. MF-exposure led to an increased phagocytic activity after 45 min, shown as a 1.6-fold increased uptake of latex beads in MF-exposed cells compared to controls. We also demonstrate an increased IL-1 beta release in macrophages after 24 h exposure (1.0 mT MF). Time-dependent IL-1 beta formation was significantly increased already after 4 h and reached a maximum of 12.3-fold increase after 24 h compared to controls. Another aspect of this study was to examine the genotoxic capacity of 1.0 mT MF by analyzing the micronucleus (MN) formation in long-term (12, 24, and 48 h) exposed macrophages. Our data show no significant differences in MN formation or irregular mitotic activities in exposed cells. Furthermore, the effects of different flux densities (ranging from 0.05 up to 1.0 mT for 45 min) of 50 Hz MF was tested on free radical formation as an endpoint of cell activation in mouse macrophage precursor cells. All tested flux densities significantly stimulated the formation of free radicals. Here, we demonstrate the capacity of ELF-EMF to stimulate physiological cell functions in mouse macrophages shown by the significantly elevated phagocytic activity, free radical release, and IL-1 beta production suggesting the cell activation capacity of ELF-EMF in the absence of any genotoxic effects.