Honeybee (Apis mellifera) Maternal Effect Causes Alternation of DNA Methylation Regulating Queen Development
Honeybee (Apis mellifera) Maternal Effect Causes Alternation of DNA Methylation Regulating Queen Development
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蜜蜂 (Apis mellifera) 母体效应导致 DNA 甲基化改变调节蜂王发育
DOI：
10.13102/sociobiology.v68i1.5935
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发表时间：
2021-03-01
期刊：
影响因子：
0.9
通讯作者：
Zeng, Zhi Jiang
中科院分区：
文献类型：
--
作者：
He, Xu Jiang;Wei, Hao;Zeng, Zhi Jiang
蜂后工蜂等级二型性是西方蜜蜂的一个典型性状。我们以前表明，种姓分化和皇后发展，皇后出现在皇后细胞卵（QE）有更高的质量比皇后从工作细胞卵（WE）的母体效应。在这项研究中，新出现的女王饲养QE，WE，和2日龄的工人幼虫（2L）。测量蚁后的胸部大小和DNA甲基化水平。我们发现，女王出现QE有显着较大的胸部长度和宽度比WE和2L。表观遗传分析显示QE/2L比较具有最多不同的甲基化基因（DMG，612），其次是WE/2L（473）和QE/WE（371）。有趣的是，大量DMG（42）位于属于mTOR、MAPK、Wnt、Notch、Hedgehog、FoxO和Hippo信号传导途径的基因中，这些信号传导途径参与调节种姓分化、生殖和寿命。本研究证明蜜蜂母体效应引起表观遗传改变，调节等级分化和蜂王发育。
Queen-worker caste dimorphism is a typical trait for honeybees (Apis mellifera). We previously showed a maternal effect on caste differentiation and queen development, where queens emerged from queen-cell eggs (QE) had higher quality than queens developed from worker cell eggs (WE). In this study, newly-emerged queens were reared from QE, WE, and 2-day worker larvae (2L). The thorax size and DNA methylation levels of queens were measured. We found that queens emerging from QE had significantly larger thorax length and width than WE and 2L. Epigenetic analysis showed that QE/2L comparison had the most different methylated genes (DMGs, 612) followed by WE/2L (473), and QE/WE (371). Interestingly, a great number of DMGs (42) were in genes belonging to mTOR, MAPK, Wnt, Notch, Hedgehog, FoxO, and Hippo signaling pathways that are involved in regulating caste differentiation, reproduction and longevity. This study proved that honeybee maternal effect causes epigenetic alteration regulating caste differentiation and queen development.