PAX3 - A PAIRED DOMAIN GENE AS A REGULATOR IN PNS MYELINATION
PAX3 - A PAIRED DOMAIN GENE AS A REGULATOR IN PNS MYELINATION
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DOI：
10.1016/0896-6273(95)90144-2
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发表时间：
1995-09-01
期刊：
影响因子：
16.2
通讯作者：
GRUSS, P
中科院分区：
文献类型：
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作者：
KIOUSSI, C;GROSS, MK;GRUSS, P
当雪旺细胞(Schwann cell，SC)前体迁移至三叉神经节时，Pax3RNA在神经嵴中表达。对小鼠坐骨神经发育和修复过程中的Pax3RNA和SC标志物进行了检测。Pax3RNA的表达与髓鞘碱性蛋白(MBP)呈负相关。在SC原代培养中也观察到负相关。腺苷环化酶激动剂Forsklin可抑制Pax3RNA、GFAP、NGFR、N-CAM和L1，升高MBP。随后微量注射Pax3表达载体使Pax3RNA、GFAP、NGFR、N-CAM和L1升高，并抑制MBP。因此，Pax3可能参与了分化为髓鞘干细胞的途径。在共转染实验中，Pax3抑制了1.3kb的MBP启动子片段，表明它抑制了MBP的转录。
Pax3 RNA is expressed in neural crest when Schwann cell (SC) precursors migrate to the PNS. Pax3 RNA and SC markers were monitored in sciatic nerves of mice during development and nerve repair. An inverse correlation was observed between expression of Pax3 RNA and myelin basic protein (MBP). Inverse correlation was also observed in SC primary cultures. Treating cultures with forskolin, an adenylate cyclase agonist, repressed Pax3 RNA, GFAP, NGFR, N-CAM, and L1 and elevated MBP. Subsequent microinjection with Pax3 expression vector elevated Pax3 RNA, GFAP, NGFR, N-CAM, and L1 and repressed MBP. Thus, Pax3 is likely involved in the differentiation pathway to myelinating SCs. Pax3 repressed a 1.3 kb MBP promoter fragment in cotransfection assays, suggesting that it represses MBP transcription.