The dynamics of murine mammary stem/progenitor cells.
The dynamics of murine mammary stem/progenitor cells.
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小鼠乳腺干/祖细胞的动力学
DOI：
10.1007/s11515-014-1308-0
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发表时间：
2014-06-01
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小鼠乳腺中的干/祖细胞是一个高度动态的细胞群体，在青春期负责导管的延长，在成年负责维持体内平衡，在怀孕期间负责小叶-肺泡的形成。近年来，由于这些不同的干/祖细胞被认为是乳腺癌不同亚型的起源细胞，了解乳腺内的上皮细胞层次变得尤为重要。尽管通过结合细胞表面蛋白抗体和流式细胞术来丰富和分离干/祖细胞，以及利用乳腺脂肪垫重建实验和活体标记技术鉴定具有多系分化和自我更新的干/祖细胞已经取得了重大进展，但对于这些不同的干/祖细胞在乳腺中是如何调控的仍然缺乏清楚的了解。在这里，我们讨论了目前可用于干细胞/祖细胞鉴定的不同的活体和体外方法，它们相关的注意事项，以及可能的新的层级模型，以协调由不同研究小组确定的各种假定的干细胞/祖细胞群体。
The stem/progenitor cells in the murine mammary gland are a highly dynamic population of cells that are responsible for ductal elongation in puberty, homeostasis maintenance in adult, and lobulo-alveolar genesis during pregnancy. In recent years understanding the epithelial cell hierarchy within the mammary gland is becoming particularly important as these different stem/progenitor cells were perceived to be the cells of origin for various subtypes of breast cancer. Although significant advances have been made in enrichment and isolation of stem/progenitor cells by combinations of antibodies against cell surface proteins together with flow cytometry, and in identification of stem/progenitor cells with multi-lineage differentiation and self-renewal using mammary fat pad reconstitution assay andin vivogenetic labeling technique, a clear understanding of how these different stem/progenitors are orchestrated in the mammary gland is still lacking. Here we discuss the differentin vivoandin vitromethods currently available for stem/progenitor identification, their associated caveats, and a possible new hierarchy model to reconcile various putative stem/progenitor cell populations identified by different research groups.