FOLDED GENOME OF ESCHERICHIA-COLI ISOLATED IN A PROTEIN-DNA-RNA COMPLEX
FOLDED GENOME OF ESCHERICHIA-COLI ISOLATED IN A PROTEIN-DNA-RNA COMPLEX
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DOI：
10.1073/pnas.68.1.6
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
PETTIJOH.DE
中科院分区：
文献类型：
--
作者：
STONINGT.OG;PETTIJOH.DE
大肠杆菌的DNA以含有少量蛋白质和RNA的紧凑结构被分离出来，沉降系数约为3200 S。复合物中DNA的分子量非常大（可能高于109）;蛋白质主要是核心RNA聚合酶; RNA主要是新生信使和核糖体链。含有这种复合物的溶液粘度很低;这加上它的沉降速率表明DNA处于紧密折叠的构象。DNA在暴露于RNA酶或热后展开;这表明复合物的RNA组分参与稳定结构。
The DNA ofEscherichia colihas been isolated in a compact structure containing small amounts of protein and RNA and having a sedimentation coefficient of approximately 3200 S. The molecular weight of the DNA in the complex is very large (probably higher than 109); the protein is predominantly core RNA polymerase; the RNA is chiefly nascent messenger and ribosomal chains. Solutions containing the complex have low viscosities; this plus its sedimentation rate suggest that the DNA is in a tightly folded conformation. The DNA unfolds after exposure to RNase or heat; this indicates that an RNA component of the complex is involved in stabilizing the structure.